Project: Functional Genomics of Benefecial Legume-Microbe Interactions

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Principal investigators: 

  • Rebecca Dickstein
  • Julia Frugoli
  • Maria Harrison
  • Kiran Mysore
  • Catalina Pislariu
  • Janine Sherrier
  • Michael Udvardi

Associated with:

  • University of North Texas
  • Clemson University
  • Boyce Thompson Institute
  • Noble Research Institute
  • Texas A&M International University
  • University of Delaware

Project Summary:

The project has four main objectives with individual sub-objectives, divided among seven Pis at six insitutions. These objectives include
1. Functional genomics of Symbiotic Nitrogen Fixation
a. Forward genetics for crucial symbiotic nitrogen fixation (SNF) genes (Dickstein, Mysore, Pislariu, Sherrier)
b. Identify and characterize Autoregulation of Nodulation genes involved in SNF symbiosis, through forward and reverse genetics and phenotyping (Frugoli)
c. Characterize nodule-specific transporters, via reverse genetics (Dickstein, Udvardi)
d. Evaluate nodulation phenotypes of symbiosis mutants generated in Objective 2 (Dickstein, Pislariu, Sherrier, Udvardi)
2. Functional genomics of Arbuscular Mycorrhizal (AM) symbiosis: characterize the functions of genes conserved for AM symbiosis through CRISPR/Cas9 genome editing and Tnt1 mutagenesis (Harrison)
3. Apply sequence capture technology to identify all Flanking Sequence Tags of Tnt1 and MERE1 elements in selected mutants (Mysore, Dickstein)
4. Enhance public understanding and education about legumes and symbiosis (Broader impacts, all PIs)

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