Profile – Pascal Ratet

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Name:

Pascal Ratet

Job Title:

Director of research CNRS

Email:

pascal.ratet@cnrs.fr

Phone Number:

00 33 (0)1 69153377

Biography:

EDUCATION
Thesis defence: June 1985 ParisXI Orsay, France
HDR, Novembre 1999 Université Paris XI, Orsay

PROFESSONAL QUALIFICATIONS
1986-1989: Post doc fellow Max Planck Institut, Köln, Germany
1989-1990: Group leader scientist, ORSAN company (France)
1990-2008 : Chargé de Recherche 1ere classe CNRS, ISV, Gif sur Yvette (France)
2008-2014: Directeur de recherche 2eme classe, CNRS, ISV, Gif sur Yvette (France)
2015-today: Directeur de recherche 2eme classe, CNRS, IPS2, Orsay (France)

MAIN RESEARCH ACTIVITIES
P. Ratet has pioneered the development of Tnt1 mutagenesis in Medicago together with the isolation of Medicago mutants and is recognized as an expert in legume-rhizobium interaction studies. P. Ratet research areas include:
-Development of genetic tools for Medicago truncatula: transgenic plant production and insertion mutagenesis in Medicago.
-Genetic studies of the plant microorganism interactions.
-Legume symbiotic organ identity.
-Role of immunity during symbiosis.
-Characterization of root and nodule endophytes.

Research Areas:

  • Rhizobium-legume symbiosis

Current N-related Projects:

  • Role of the plant immunity in its interaction with rhizobia

Bibliography:

– Pawlowski K., Ratet P., Schell J., de Bruijn F.J. (1987) Cloning and characterization of nifA and ntrC genes of the stem nodulating bacterium ORS571, the nitrogen fixing symbiont of Sesbania rostrata : Regulation of nitrogen fixation (nif) genes in the free living versus symbiotic state. Mol. Gen. Genet., 206, 207-219.
– Ratet P., Pawlowski K., Meyer H., Schell J., de Bruijn F.J. (1987) Regulation of nitrogen fixation (nif) genes of Azorhizobium caulinodans ORS571 in culture and in planta. J. Plant Physiol., 132, 405-411.
– Murphy P.J., Heyke N., Trenz S.P., Ratet P., F.J. de Bruijn, Schell J. (1988) Synthesis of an opine-like compound, a rhizopine, in alfalfa nodules is symbiotically regulated. Proc. Natl. Acad. Sci. U.S.A., 85, 9133-9137.
– Ratet P., Schell J., de Bruijn F.J. (1988) Mini-Mu-lac transposons with broad-host-range origins of conjugal transfer and replication designed for gene regulation studies in Rhizobiaceae. Gene, 63, 41-52.
– de Bruijn F.J., Felix G., Grunenberg B., Hoffmann H.J., Metz B., Ratet P., Simons-Schreier A., Szabados L., Welters P., Schell J. (1989) Regulation of plant genes specifically induced in nitrogen-fixing nodules: role of cis-acting elements and trans-acting factors in leghemoglobin gene expression. Plant Mol. Biol., 13, 319-325.
– de Bruijn F.J., Rossbach S., Schneider M., Ratet P., Messner S., Szeto W.W., Ausubel F., Schell J. (1989) Rhizobium meliloti 1021 has three differentially regulated loci involved in glutamine synthesis, none of which is essential for symbiotic nitrogen fixation. J. Bacteriol., 171, 1673-1682.
– Ratet P., Pawlowski K., Schell J., de Bruijn F.J. (1989) The Azorhizobium caulinodans nitrogen fixation regulatory gene nifA is controlled by cellular nitrogen and oxygen status. Mol. Microbiol., 6, 825-838.
– Szabados L., Ratet P., Grunenberg B., de Bruijn F.J. (1990) Functional analysis of the Sesbania rostrata leghemoglobin glb3 gene 5′-upstream region in transgenic Lotus corniculatus and Nicotiana tabacum plants. The Plant Cell, 2, 973-986.
– Bauer P., Crespi M.D., Szécsi J., Allison L., Schultze M., Ratet P., Kondorosi E., Kondorosi A. (1994) Alfalfa Enod12 genes are differentially regulated during nodule development by Nod factors and Rhizobium invasion. Plant Physiol., 105, 585-592.
– Coronado C., Zuanazzi J.A.S., Sallaud C., Quirion J-C., Esnault R., Husson H-P., Kondorosi A., Ratet P. (1995) Alfalfa root flavonoid production is nitrogen regulated. Plant Physiol., 108, 533-542.
– Bauer, P., Ratet P., Crespi, M.D., Schultze, M. and Kondorosi, A. (1996) Nod factors and cytokinins induce similar patterns of cortical cell division, MsEnod12A expression and amyloplast deposition in alfalfa roots. Plant J., 10, 91-105.
– Bauer P., Poirier S., Ratet P., Kondorosi A. (1997) MsEnod12A expression is linked to meristematic activity during development of indeterminate and determinate nodules and roots. Mol. Plant-Microbe Interact., 10, 39-49.
– Carrayol E., Desbrosses G., Pruvot-Maschio G., Poirier S., Ratet P., Hirel B. (1997) Ammonia regulated expression of a soybean gene encoding cytosolic glutamine synthase is not conserved in two heterologous plant systems. Plant Sci., 125, 75-85.
– Savouré A., Sallaud C., El Turk J., Zuanazzi J., Ratet P., Schultze M., Kondorosi A., Esnault R., Kondorosi E. (1997) Distinct response of Medicago suspension cultures and roots to Nod factors and chitin oligomers in the elicitation of defense-related responses. Plant J., 11, 277-287.
– Charrier B., Trinh H., Poirier S., Kondorosi A., Ratet P. (1998) Flavanone 3-hydroxylase (F3H) expression and flavonoid localization in nodules of three legume plants reveal distinct tissue specificities. Mol. Plant-Microbe Interact., 11, 924-932.
– Munoz J.A., Coronado C., Pérez-Hormaeche J., Kondorosi A., Ratet P., Palomares A.J. (1998) MsPG3, a Medicago sativa polygalacturonase gene expressed during the alfalfa-Rhizobium meliloti interaction. Proc. Natl. Acad. Sci. USA, 95, 9687-9692.
– Trinh T.H., Ratet P., Kondorosi E., Durand P., Kamate K., Bauer P., Kondorosi A. (1998) Rapid and efficient transformation of diploid Medicago truncatula and Medicago sativa ssp. falcata lines improved in somatic embryogenesis. Plant Cell Rep., 17, 345-355.
– Zuanazzi J.A.S., Clergeot P.H., Quirion J.C., Husson H-P., Kondorosi A., Ratet P. (1998) Production of Sinorhizobium meliloti nod gene activator and repressor flavonoids from Medicago sativa roots. Mol. Plant-Microbe Interact., 11, 784-794.
– d’Erfurth I., Cosson V., Eschstruth, A., Lucas, H., Kondorosi A., Ratet P. (2003) Efficient transposition of the Tnt1 tobacco retrotransposon in the model legume Medicago truncatula. Plant J., 34, 95-106.
– Rodriguez-Llorente I.D., Pérez-Hormaeche J., Dary M., Caviedes M.A., Trinh, T.H., Kondorosi A., Ratet P., Palomares A.J. (2003) MsPG3 promoter elements necessary for expression during Sinorhizobium meliloti-Medicago truncatula interaction. Plant and Soil, 257, 19-26.
– Rodriguez-Llorente I.D., Pérez-Hormaeche J., El Mounadi K., Dary M., Caviedes M.A., Cosson V., Kondorosi A., Ratet P., Palomares A.J. (2004) From Pollen tubes to infection thread: recruitement of Medicago floral pectic genes for symbiosis. Plant J., 39, 587-598.
– Tadege M., Ratet P., Mysore K.S. (2005) Insertional mutagenesis: a Swiss army knife for functional genomics of M. truncatula. Trends in plant Sciences, 10 (5), 229-235.
– Marsh J.F., Rakocevic A., Mitra R. M., Brocard L., Sun J., Eschstruth A., Long S.R., Schultze M., Ratet P. Oldroyd G.E.D. (2007) Medicago truncatula NIN is essential for Rhizobial-independent nodule organogenesis induced by autoactive calcium/calmodulin-dependent protein kinase. Plant Physiol. 144, 324-335.
– Porceddu A., Panara F., Calderini O., Molinari L., Taviani P., Lanfaloni L., Scotti C., Carelli M., Scaramelli L., Bruschi G., Cosson V., Ratet P., de Larembergue H., Duc G., Piano E. Arcioni S. (2008) An Italian functional genomic resource for Medicago truncatula. BMC Research Notes, 1:129
– Iantcheva A., Chabaud M., Cosson V., Barascud M., Schutz B., Primard-Brisset C., Durand P., Barker D.G., Atanasov A., Vlahova M., Ratet P. (2009) Osmotic shock improves Tnt1 transposition frequency in Medicago truncatula cv Jemalong during in vitro regeneration. Plant Cell Rep. 28 (10) : 1563-72. Epub 2009 Aug 18.
– Tadege M, Wang TL, Wen J, Ratet P, Mysore KS. (2009) Mutagenesis and Beyond! Tools for understanding legume biology. Plant Physiol. 151(3):978-84. Epub 2009 Sep 9.
– Maunoury N, Redondo-Nieto M, Bourcy M, Van de Velde W, Alunni B, Laporte P, Durand P, Agier N, Marisa L, Vaubert D, Delacroix H, Duc G, Ratet P, Aggerbeck L, Kondorosi E, Mergaert P. (2010) Differentiation of symbiotic cells and endosymbionts in Medicago truncatula nodulation are coupled to two transcriptome-switches. PLoS One. 2010 Mar 4;5(3):e9519.
– Laffont C, Blanchet S, Lapierre C, Brocard L, Ratet P, Crespi M, Mathesius U, Frugier F. (2010) The compact root architecture1 gene regulates lignification, flavonoid production, and polar auxin transport in Medicago truncatula. Plant Physiol. 153(4):1597-607. Epub 2010 Jun 3.
– Murray J.D., Duvvuru Muni R.S.R., Torres-Jerez I., Tang Y., Allen S., Andriankaja M., Li G., Laxmi A., Cheng X., Wen J., Vaughan D., Schultze M., Sun, J, Charpentier M., Oldroyd G., Tadege M., Ratet P., Mysore K. S., Chen R. and Udvardi M.K. (2011) Vapyrin, a gene essential for intracellular progression of arbuscular mycorrhizal symbiosis, is also essential for infection by rhizobia in the nodule symbiosis of Medicago truncatula. Plant J. 65, 244-252.
– Horváth B., Yeun L. H., Domonkos Á., Halász G., Gobbato E., Ayaydin F., Míró K., Hirsch S., Sun J., Tadege M., Ratet P., Mysore K., Ané J.-M., Oldroyd G. E. D. and Kaló P. (2011) Medicago truncatula IPD3 is a member of the common symbiotic signaling pathway required for rhizobial and mycorrhizal symbioses. Mol Plant Microbe Interact. 24(11), 1345-1358.
-Ovchinnikova E., Journet E.-P., Chabaud M., Cosson V., Ratet P., Duc G., Fedorova E., Liu W., Op den Camp R., Zukov V., Tikhovovich I., Borisov A., Bisseling T., Limpens E. (2011) IPD3, controls the intracellular accommodation of rhizobia in pea and Medicago. Mol Plant Microbe Interact. 24(11), 1333-1344.
– Revalska M, Vassileva V, Goormachtig S, Van Hautegem T, Ratet P, Iantcheva A. (2011)
Recent Progress in Development of Tnt1 Functional Genomics Platform for Medicago truncatula and Lotus japonicus in Bulgaria. Curr Genomics. 12(2):147-152.
– Pislariu CI, Murray J, Wen J, Cosson V, Duvvuru Muni RR, Wang M, Benedito V, Andriankaja A, Cheng X, Torres Jerez I, Mondy S, Zhang S, Taylor M, Tadege M, Ratet P, Mysore KS, Chen R, Udvardi MK. (2012) A Medicago truncatula tobacco-retrotransposon (Tnt1)-insertion mutant collection with defects in nodule development and symbiotic nitrogen fixation. Plant Physiol. 159(4): 1686-99.
– Couzigou J.M., Zhukov V., Mondy S., Abu el Heba G., Cosson C., Ellis T.H.N., Ambrose M., Wen J., Tadege M., Tikhonovich I., Mysore S.K., Putterill J., Hofer J., Borisov A.Y., and Ratet P. (2012) NODULE ROOT and COCHLEATA maintain nodule development and are legume orthologs of Arabidopsis BLADE-ON-PETIOLE genes. Plant Cell, 24(11), 4498-4510.
– Bourcy M., Brocard L., Pislariu C.I., Cosson V., Mergaert P., Tadege M., Mysore K.S., Udvardi M.K., Gourion B., Ratet P. (2013) Medicago truncatula DNF2 is a PI-PLC-XD-containing protein required for bacteroid persistence and prevention of nodule early senescence and defense-like reactions. New Phytologist, 197(4), 1250-61.
– Bourcy M, Berrabah F, Ratet P, Gourion B. (2013) Failure of self-control : Defense like reactions during legume/rhizobia symbiosis. Plant Signal Behav. 2013 Feb 20;8(4). doi:pii: e23915. [Epub ahead of print]
– Couzigou JM, Mondy S., Sahl L., Gourion B., Ratet P. (2013) To be or noot to be: evolutionary tinkering for symbiotic organ identity. Plant Signal Behav. May 15;8(8). doi:pii: e24969. [Epub ahead of print]
– Iantcheva A., Mysore K.S., Ratet P. (2013) Transformation of leguminous plants to study symbiotic interactions. Int. J. Dev. Biol. 57(6-8): 577-586.
– Cheng X., Wang M., Lee H.K., Tadege M., Ratet P., Udvardi M., Mysore K.S. Wen J., (2014) An efficient reverse genetics platform in the model legume Medicago truncatula. New Phytologist, 201(3):1065-76. doi: 10.1111/nph.12575. Epub 2013 Nov 11.
– Berrabah F., Bourcy M., Cayrel A., Eschstruth A., Mondy S., Ratet P., Gourion B. (2014) Growth Conditions Determine the DNF2 Requirement for Symbiosis. PLoS ONE 9(3): e91866. doi:10.1371/journal.pone.0091866
– Berrabah F., Bourcy M., Eschstruth A., Cayrel A., Guefrachi I., Mergaert P., Wen J., Cosson V., Mysore K.S, Gourion B., Ratet P. (2014) A non-RD receptor-like kinase prevents nodule early senescence and defense-like reactions during symbiosis. New Phytologist, 203(4): 1305-14. doi: 10.1111/nph.12881. [Epub ahead of print].
– Guefrachi I., Nagymihaly M., Pislariu C.I., Van de Velde W., Ratet P., Mars M., Udvardi M.K., Kondorosi E., Mergaert P., Alunni B. (2014) Extreme specificity of NCR gene expression in Medicago truncatula. BMC Genomics, 15:712. doi:10.1186/1471-2164-15-712
– Huault E, Laffont C, Wen J, Mysore KS, Ratet P, Duc G, Frugier F. (2014) Local and systemic regulation of plant root system architecture and symbiotic nodulation by a receptor-like kinase. PLoS Genet. Dec 18;10(12):e1004891. doi: 10.1371/journal.pgen.1004891. eCollection 2014 Dec.
– Gourion B, Berrabah F, Ratet P, Stacey G. (2015) Rhizobium-legume symbioses: the crucial role of plant immunity. Trends Plant Sci. 20 (3): 186-94. doi: 10.1016/j.tplants.2014.11.008. [Epub ahead of print] Review.
– Berrabah F, Ratet P, Gourion B. (2015) Multiple steps control immunity during the intracellular accommodation of rhizobia. J Exp Bot. 66(7): 1977-85. doi: 10.1093/jxb/eru545. Epub 2015 Feb 14.
– Chaintreuil C, Rivallan R, Bertioli DJ, Klopp C, Gouzy J, Courtois B, Leleux P, Martin G, Rami JF, Gully D, Parrinello H, Séverac D, Patrel D, Fardoux J, Ribière W, Boursot M, Cartieaux F, Czernic P, Ratet P, Mournet P, Giraud E, Arrighi JF. (2016) A gene-based map of the Nod factor-independent Aeschynomene evenia sheds new light on the evolution of nodulation and legume genomes. DNA Res. 23 (4): 365-376. doi: 10.1093/dnares/dsw020.
– Hamoum H., Bouznad A., Mekhaldi A., Ratet P., Djibaoui R. (2016) Free-living diazotrophic rhizobacteria with plant growth promoting traits isolated from the rhizosphere of wheat grown on saline soil in west Algeria. J. Appl. Environ. Biol. Sci. 6(11), 1-10.
– Brusamarello-Santos LC, Gillard F, Brulé L, Quilleré I, Gourion B, Ratet P, de Souza EM, Lea PJ, Hirel B (2017) Metabolic profiling of two maize (Zea mays l.) inbred lines inoculated with the nitrogen fixing plant-interacting bacteria Herbaspirillum seropedicae and Azospirillum brasilense. PLoS ONE 12(3): e0174576. https://doi.org/10.1371/ journal.pone.0174576.