Profile – José-María Vinardell

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José-María Vinardell

Job Title:

Associate Professor


Phone Number:

+34 954554330


Google Scholar Profile


Dr. José-María Vinardell
Associate professor at Department of Microbiology, Faculty of Biology, University of Sevilla (Spain)
PhD in Biology. University of Seville (1997)
Postdoctoral stay at Eva Kondorosi’s lab, Institut des Sciences Végétales (ISV-CNRS), Gif-sur-Yvette, France. 1998-2000.
Domains of expertise : rhizobial-legume symbiosis, bacterial genomics, rhizobial surface polysaccharides, rhizobial molecular signals
Training of 5 PhD students
Bibliometry (web of science): 47 peer-reviewed publications, h-index: 21. External citations: 909. Average citations per item: 23.

Research Areas:

  • Biological nitrogen fixation
  • Sinorhizobium fredii/Glycine max symbiosis
  • Rhizobial genomics
  • Rhizobial molecular signals

Current N-related Projects:

  • The Sinorhizobum fredii symbiotic regulon: identification of key genes involved in the symbiotic interaction with soybean and with the model legume Lotus


1) Vinardell, J.M.; Buendía-Clavería, A & J.E. Ruiz-Sainz. 1993. A method for the positive selection of spontaneous Rhizobium mutants showing transcriptional activation of nodulation genes in the absence of nod-inducers. Molecular Plant-Microbe Interactions 6: 782-785.
2) Cubo, T.; Romero, F.; Vinardell, J.M., and Ruiz-Sainz, J.E. 1997. Expression of the Rhizobium leguminosarum biovar phaseoli melA gene does not require the presence of the nifA gene in other rhizobia. Australian Journal of Plant Physiology 24:195-205.
3) Vinardell, J.M.; Ollero, F.J.; Krishnan, H.B.; Espuny, M.R.; Villalobo, E.; Pueppke, S.G., and Ruiz-Sainz, J.E. 1997. ISRf1, a transposable insertion sequence from Sinorhizobium fredii. Gene 204:63-69.
4) Machado, D.; Pueppke, S.G.; Vinardell, J.M.; Ruiz-Sainz, J.E., and Krishnan, H.B. 1998. Expression of nodD1 and nodD2 in Sinorhizobium fredii, a nitrogen-fixing symbiont of soybean and other legumes. Molecular Plant-Microbe Interactions 11: 375-382.
5) Lamrabet, Y.; Bellogín, R.A.; Cubo, T.; Espuny, R.; Gil, A.; Krishnan, H.B.; Megías, M.; Ollero, F.J.; Pueppke, S.G.; Ruiz-Sainz, J.E.; Spaink, H.P.; Tejero-Mateo, P.; Thomas-Oates, J., and Vinardell, J.M. 1999. Mutation in GDP-fucose synthesis genes of Sinorhizobium fredii alters Nod factors and significantly decreases competitiviness to nodulate soybeans. Molecular Plant-Microbe Interactions 12: 207-217.
6) Cebolla, A.; Vinardell, J.M.; Kiss, E.; Oláh, B.; Roudier, F.; Kondorosi, A., and Kondorosi; E. 1999. The mitotic inhibitor ccs52 is required for endoreduplication and ploidy-dependent cell enlargement in plants. The EMBO Journal 18: 4476-4484.
7) Yang, S.S., Bellogín, R., Buendía, A., Camacho, M., Chen, M.; Cubo, T., Daza, A., Díaz, C.L., Espuny, M.R., Gutiérrez, R., Harteveld, M., Li, X.H., Lyra, M.C.C.P., Madinabeitia, N., Medina, C., Miao, L., Ollero, F.J., Olsthoorn, M.M.A., Rodríguez, D.N., Santamaría, C., Schlaman, H.R.M., Spaink, H.P.,Temprano, F., Thomas-Oates, J.E., van Brussel, A.A.N., Vinardell, J.M., Xie, F., Yang, J., Zhang, H.Y., Zhen, J., Zhou, J. , and Ruiz-Sainz, J.E. 2001. Effect of pH and soybean cultivars on the quantitative analyses of soybean rhizobia populations. Journal of Biotechnology 91: 243-255.
8) Madinabeitia, N., Bellogín, R.A., Buendía-Clavería, A., Camacho, M., Cubo, T., Espuny, M.R., Gil-Serrano, A.M., Lyra, M.C.C.P., Moussaid, A., Ollero, F.J., Soria-Díaz, M.E., Vinardell, J.M., Zeng, J., and Ruíz-Sainz, J.E. 2002. Sinorhizobium fredii HH103 has a truncated nolO gene due to a –1 frameshift mutation that is conserved among other geographically distant S. fredii strains. Molecular Plant-Microbe Interactions 15: 150-159.
9) Kevei, Z., Vinardell, J.M., Kiss, G.B., Kondorosi, A., and Kondorosi, E. 2002. Glycine-rich proteins encoded by a nodule-specific gene family are implicated in different stages of symbiotic nodule development in Medicago Spp. Molecular Plant-Microbe Interactions 15: 922-931.
10) Favery, B., Complainville, A., Vinardell, J.M., Lecomte, P., Vaubert, D., Mergaert, P., Kondorosi, A., Kondorosi, E., Crespi, M., and Abad, P. 2002. The Endosymbiosis-Induced Genes ENOD40 and CCS52a Are Involved in Endoparasitic-Nematode Interactions in Medicago truncatula. Molecular Plant-Microbe Interactions 15: 1008-1013.
11) Camacho M., Santamaría C., Temprano F., Rodríguez-Navarro D.N., Daza A., Espuny R., Bellogín R., Ollero F.J., Lyra De M.C.C.P., Buendía-Clavería A., Zhou J., Li F.D., Mateos C., Velázquez E., Vinardell J.M., and Ruiz-Sainz J.E. 2002. Soils of the Chinese Hubei province show a very high diversity of Sinorhizobium fredii strains. Systematic and Applied Microbiology 25: 592-602.
12) Rodríguez-Navarro, D.N., Bellogín, R., Camacho, M., Daza, A., Ollero, F.J., Santamaría, C., Medina, C., Vinardell, J.M., Ruiz-Sainz, J.E., and Temprano, F.J. 2003. Field assessment and genetic stability of Sinorhizobium fredii strain SMH12 for commercial soybean inoculants. European Journal of Agronomy 19: 301-313.
13) Buendía-Clavería, A.M., Moussaid, A., Ollero, F.J., Vinardell, J.M., Torres, A., Moreno, J., Gil-Serrano, A.M., Rodríguez-Carvajal, M.A., Tejero-Mateo, P., Peart, J.L., Brewin, N.J., and Ruiz-Sainz, J.E. 2003. A purL mutant of Sinorhizobium fredii HH103 is symbiotically defective and altered in its lipopolysaccharide. Microbiology 149: 1807-1818.
14) Saldaña, G., Martinez-Alcántara, V., Vinardell, J.M, Bellogín, R., Ruíz-Sainz, J.E., and Balatti, P.A. 2003. Genetic diversity of fast-growing rhizobia that nodulate soybean (Glycine max L. Merr). Archives of Microbiology 180: 45-52.
15) Thomas-Oates, J., Bereszczak, J., Edwards, E., Gill, A., Noreen, S., Zhou, J.C., Chen, M.Z., Miao, L.H., Xie, F.L., Yang, J.K., Zhou, Q., Yang, S.S., Li, X.H., Wang, L., Spaink, H.P., Schlaman, H.R.M., Harteveld, M., Díaz, C.L., van Brussel, A.A.N., Camacho, M., Rodríguez-Navarro, D.N., Santamaría, C., Temprano, F., Acebes, J.M., Bellogín, R.A., Buendía-Clavería, A.M., Cubo, M.T., Espuny, M.R., Gil, A.M., Gutiérrez, R., Hidalgo, A., López-Baena, F.J., Madinabeitia, N., Medina, C., Ollero, F.J., Vinardell, J.M., and Ruiz-Sainz, J.E. 2003. A catalogue of molecular, physiological and symbiotic properties of soybean-nodulating rhizobial strains from different soybean cropping areas of China. Systematic and Applied Microbiology 26: 453-465.
16) Vinardell, J.M., Federova, E., Cebolla, A., Kevei, Z., Horvarth, G., Kelemen, Z., Tarayre, S., Roudier, F., Mergaert, P., Kondorosi, A., and Kondorosi, E. 2003. Endoreduplication mediated by the Anaphase-Promoting Complex activator CCS52A is required for symbiotic cell differentiation in Medicago truncatula nodules. The Plant Cell 15: 2093-2105.
17) Noreen, S., Schlaman, H.R.M., Bellogín, R.A., Buendía-Clavería, A.M., Espuny, M.R., Harteveld, M., Medina, C., Ollero, F.J., Olsthoorn, M.M.A., Soria-Diaz, M.E., Spaink, H.P., Temprano, F., Thomas-Oates, J., Vinardell, J.M., Yang, S.S., Zhang, H., and Ruiz-Sainz, J.E. 2003. Alfalfa nodulation by S. fredii does not require sulfated Nod-factors. Functional Plant Biology 30: 1219-1232.
18) Tarayre, S., Vinardell, J.M., Cebolla, A., Kondorosi, A., and Kondorosi, E. 2004. Two classes of the Cdh1-type activators of the Anaphase-Promoting Complex in plants: novel functional domains and distinct regulation. The Plant Cell 16: 422-434.
19) Vinardell, J.M., López-Baena, F.J., Hidalgo, A., Ollero, F.J., Bellogín, R., Espuny, M.R., Temprano, F., Romero, F., Krishnan, H.B., Pueppke, S.G., and Ruiz-Sainz, J.E. 2004. The effect of FITA mutations on the symbiotic properties of S. fredii varies in a chromosomal-background-dependent manner. Archives of Microbiology 181: 144-154.
20) Vinardell, J.M., Ollero, F.J., Hidalgo, A., López-Baena, F.J., Medina, C., Ivanov-Vangelov, K., Parada, M., Madinabeitia, N., Espuny, M.R., Bellogín, R.A., Camacho, M., Rodríguez-Navarro, D.N., Soria-Díaz, M.E., Gil-Serrano, A.M., and Ruiz-Sainz, J.E. 2004. NolR regulates diverse symbiotic signals of Sinorhizobium fredii HH103. Molecular Plant-Microbe Interactions 17: 676-685.
21) Parada, M., Vinardell, J.M., Ollero, F.J., Hidalgo, A., Guitiérrez, R., Buendía-Clavería, A.M., Lei, W., Margaret, I., López-Baena, F.J., Gil-Serrano, A.M., Rodríguez-Carvajal, M.A., Moreno, J., Ruiz-Sainz, J.E. 2006. Sinorhizobium fredii HH103 mutants affected in capsular polysaccaride (KPS) are impaired for nodulation with soybean and Cajanus cajan. Molecular Plant-Microbe Interactions 19: 43-52.
22) de Lyra, M.C.C.P., López-Baena, F.J., Madinabeitia, N., Vinardell, J.M., Espuny, M.R., Cubo, M.T., Bellogín, R.A., Ruiz-Sainz, J.E., Ollero, F.J. 2006. Inactivation of the Sinorhizobium fredii HH103 rhcJ gene abolishes the secretion of nodulation outer proteins and decreases the symbiotic capacity with soybean. International Microbiology 9: 125-133.
23) Rodrigues, J.A., López-Baena, F.J., Ollero, F.J., Vinardell, J.M., Espuny, M.R., Bellogín, R.A., Ruiz-Sainz, J.E., Thomas, J.R., Sumpton, D., Ault, J., Thomas-Oates, J. 2007. NopM and NopD are rhizobial nodulation outer proteins: identification using LC-MALDI and LC-ESI with a monolithic capillary column. Journal of Proteome Research 6: 1029-1037.
24) Monreal, J.A., Feria, A.B., Vinardell, J.M., Vidal, J., Echevarría, C., García-Mauriño, S. 2007. ABA modulates the degradation of phosphoenolpyruvate carboxylase kinase in sorghum leaves. FEBS Letters 581: 3498-3472.
25) Crespo-Rivas, J.C., Margaret, I., Pérez-Montaño, F., López-Baena, F.J., Vinardell, J.M., Ollero, F.J., Moreno, J., Ruiz-Sainz, J.E., Buendía-Clavería, A.M. 2007. A pyrF auxothrophic mutant of Sinorhizobium fredii HH103 impaired in its symbiotic interactions with soybean and other legumes. International Microbiology 10: 169-176.
26) López-Baena, F.J., Vinardell, J.M., Pérez-Montaño, F., Crespo-Rivas, J.C., Bellogín, R.A., Espuny, M.R., Ollero, F.J. 2008. Regulation and symbiotical significance of nodulation outer proteins secretion in Sinorhizobium fredii HH103. Microbiology 154: 1825-1836.
27) Crespo-Rivas, J.C., Margaret, I., Hidalgo, Á., Buendía-Clavería, A.M., Ollero, F.J., López-Baena, F.J., Murdoch, P.S., Rodríguez-Carvajal, M.A., Soria-Díaz, M.E., Reguera, M., Lloret, J., Sumpton, D.P., Mosely, J.A., Thomas-Oates, J.E., van Brussel, A.A.N., Gil-Serrano, A., Vinardell, JM., Ruiz-Sainz, JE. 2009. Sinorhizobium fredii HH103 cgs mutants are unable to nodulate determinate- and indeterminate-nodule forming legumes and overproduce an altered EPS. Molecular Plant-Microbe Interactions 22: 575-588.
28) López-Baena, F.J., Monreal, J.A., Pérez-Montaño, F., Guasch-Vidal, B., Bellogín, R.A., Vinardell, J.M., Ollero, F.J. 2009. The absence of Nops secretion in Sinorhizobium fredii HH103 increases GmPR1 expression in Williams soybean. Molecular Plant-Microbe Interactions 22: 1445-1454.
29) Reguera, M., Lloret, J., Margaret, I., Vinardell, J.M., Martín, M., Buendía, A., Rivilla, R., Ruiz-Sainz, J.E., Bonilla, I., Bolaños, L. 2009. Gene SMb21071 of plasmid pSymB is required for osmoadaptation of Sinorhizobium meliloti 1021 and is implicated in modifications of cell surface polysaccharides structure in response to hyperosmotic stress. Canadian Journal of Microbiology 55: 1145-1152.
30) Hidalgo A, Margaret I, Crespo-Rivas JC, Parada M, Murdoch Pdel S, López A, Buendía-Clavería AM, Moreno J, Albareda M, Gil-Serrano AM, Rodríguez-Carvajal MA, Palacios JM, Ruiz-Sainz JE, Vinardell JM. 2010. The rkpU gene of Sinorhizobium fredii HH103 is required for bacterial K-antigen polysaccharide production and for efficient nodulation with soybean but not with cowpea. Microbiology 156: 3398-3411.
31) Margaret I, Becker A, Blom J, Bonilla I, Goesmann A, Göttfert M, Lloret J, Mittard-Runte V, Rückert C, Ruiz-Sainz JE Vinardell JM, Weidner S. 2011. Symbiotic properties and genomic sequence of the fast growing model strain Sinorhizobium fredii HH103 nodulating soybean. Journal of Biotechnology 155: 11-19.
32) Margaret Oliver I, Lei W, Parada M, Rodríguez-Carvajal MA, Crespo-Rivas JC, Hidalgo A, Gil-Serrano A, Moreno J, Rodríguez-Navarro DN, Buendía-Clavería A, Ollero J, Ruiz-Sainz JE, Vinardell JM. 2012. Sinorhizobium fredii HH103 does not strictly require KPS and/or EPS to nodulate Glycyrrhiza uralensis, an indeterminate nodule-forming legume. Archives of Microbiology 194: 87-102.
33) Abarca-Grau AM, Burbank LP, de Paz HD, Crespo-Rivas JC, Marco-Noales E, López MM, Vinardell JM, von Bodman SB, Penyalver R. 2012. Role for Rhizobium rhizogenes K84 cell envelope polysaccharides in surface interactions. Applied and Environmental Microbiology 78: 1644-1651.
34) Weidner S, Becker A, Bonilla I, Jaenicke S, Lloret J, Margaret I, Pühler A, Ruiz-Sainz JE, Schneiker-Bekel S, Szczepanowski R, Vinardell JM, Zehner S, Göttfert M. 2012. Genome sequence of the soybean symbiont Sinorhizobium fredii HH103. Journal of Bacteriology 194: 1617-1618.
35) Margaret I, Crespo-Rivas JC, Acosta-Jurado S, Buendía-Clavería AM, Cubo MT, Gil-Serrano A, Moreno J, Murdoch PS, Rodríguez-Carvajal MA, Rodríguez-Navarro DN, Ruiz-Sainz JE, Sanjuán J, Soto MJ, Vinardell JM. 2012. Sinorhizobium fredii HH103 rkp-3 Genes Are Required for K-Antigen Polysaccharide Biosynthesis, Affect Lipopolysaccharide Structure and Are Essential for Infection of Legumes Forming Determinate Nodules. Molecular Plant-Microbe Interactions 25: 825-838.
36) Margaret I, Lucas MM, Acosta-Jurado S, Buendía-Clavería AM, Federova E, Gil-Serrano A, Hidalgo A, Moreno J, Rodríguez-Carvajal MA, Rodríguez-Navarro DN, Ruiz-Sainz JE, Vinardell JM. 2013. The Sinorhizobium fredii HH103 lipopolysaccharide is not only relevant at early soybean nodulation stages but also for symbiosome stability in mature nodules. PLoS ONE 8(10): e74717. doi:10.1371/journal.pone.0074717
37) Rodríguez-Navarro DN, Rodríguez-Carvajal MA, Acosta-Jurado S, Soto MJ, Margaret I, Crespo-Rivas JC, Sanjuan, J, Temprano F, Gil-Serrano A, Ruiz-Sainz JE, Vinardell JM. 2014. Structure and biological roles of Sinorhizobium fredii HH103 exopolysaccharide. PLoS ONE 9(12): e115391.doi:10.1371/journal.pone.0115391
38) Vinardell JM, Acosta-Jurado S, Göttfert M, Zehner S, Becker A, Baena-Ropero I, Blom J, Crespo-Rivas JC, Goesmann A, Jaenicke S, Krol E, McIntosh M, Margaret I, Pérez-Montaño F, Schneiker-Bekel S, Serrania J, Szczepanowski R, Buendia-Claveria AM, Lloret J, Bonilla I, Pühler A, Ruiz-Sainz JE, Weidner S. 2015. The Sinorhizobium fredii HH103 genome: a comparative analysis with S. fredii strains differing in their symbiotic behaviour with soybean. Molecular Plant-Microbe Interactions 28:811-824.
39) Crespo-Rivas JC, Guefrachi I, Mok KC, Villaécija-Aguilar JA, Acosta-Jurado S, Pierre O, Taga ME, Mergaert P, Vinardell JM. 2016. Sinorhizobium fredii HH103 bacteroids are not terminally differentiated and show altered O-antigen in nodules of the IRLC legume Glycyrrhiza uralensis. Environ. Microbiol. 18:2392-2404.
40) López-Baena FJ, Ruiz-Sainz JE, Rodríguez-Carvajal MA, Vinardell JM. Molecular signals in the Sinorhizobium fredii soybean symbiosis. 2016. Int. J. Mol. Sci. 17: pii: E755.
41) Pérez-Montaño F, Jiménez-Guerrero I, Acosta-Jurado S, Navarro-Gómez P, Ollero FJ, Ruiz-Sainz JE, López-Baena FJ, Vinardell JM. 2016. A transcriptomic analysis of the effect of genistein on Sinorhizobium fredii HH103 reveals novel rhizobial genes putatively involved in symbiosis. Sci. Rep. 6:31592.
42) Acosta-Jurado S, Navarro-Gómez P, Murdoch PdelS, Crespo-Rivas JC, Jie S, Cuesta-Berrio L, Ruiz-Sainz JE, Rodríguez-Carvajal MA, Vinardell JM. 2016. Exopolysaccharide production by Sinorhizobium fredii HH103 is repressed by genistein in a NodD1-dependent manner. PLoS One. 11:e0160499.
43) Acosta-Jurado S, Alias-Villegas C, Navarro-Gómez P, Zehner S, Murdoch PD, Rodríguez-Carvajal MA, Soto MJ, Ollero FJ, Ruiz-Sainz JE, Göttfert M, Vinardell JM. 2016. The Sinorhizobium fredii HH103 MucR1 Global Regulator Is Connected With the nod Regulon and Is Required for Efficient Symbiosis With Lotus burttii and Glycine max cv. Williams. Mol Plant Microbe Interact. 29:700-712.
44) Acosta-Jurado S, Rodríguez-Navarro DN, Kawaharada Y, Perea JF, Gil-Serrano A, Jin H, An Q, Rodríguez-Carvajal MA, Andersen SU, Sandal N, Stougaard J, Vinardell JM, Ruiz-Sainz JE. 2016. Sinorhizobium fredii HH103 invades Lotus burttii by crack entry in a Nod Factor-and Surface Polysaccharide-dependent manner. Mol Plant Microbe Interact. 29:925-937.
45) Toro N, Villadas PJ, Molina-Sánchez MD, Navarro-Gómez P, Vinardell JM, Cuesta-Berrio L, Rodríguez-Carvajal MA. 2017. The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population. Sci. Rep. 7:675.
46) Acosta-Jurado S, Navarro-Gómez P, Crespo-Rivas JC, Medina C, Murdoch PS, Cuesta-Berrio L, Rodríguez-Carvajal MA, Ruiz-Sainz JE, Vinardell JM. 2017. The Sinorhizobium (Ensifer) fredii HH103 rkp-2 region is involved in the biosynthesis of lipopolysaccharide and exopolysaccharide but not in K-antigen polysaccharide production. Plant and Soil 417: 415-431.
47) Hidalgo A, López-Baena FJ, Ruiz-Sainz JE, Vinardell JM. 2017. Studies of rhizobial competitiveness for nodulation in soybean using a non-destructive split-root system. AIMS Microbiol 3:323-334.