Profile – Jacques Batut



Jacques Batut

Job Title:

Senior scientist at INRA


Phone Number:

33 5 61 28 50 54


Dr Jacques BATUT
Laboratory of Plant-Microorganism Interactions (LIPM) CNRS-INRA Toulouse France
Researcher in C. Masson’s group « Symbiotic functions, genomes and evolution of rhizobia »
PhD in Microbiology. University Toulouse III (1984)
Sabbatical at UC Berkeley (Prof S. Kustu) (1989-1990)
Director of Research (DR1) at the Institut National de la Recherche Agronomique, France (2005-)
Domains of expertise : symbiotic nitrogen fixation, symbioses, plant-microbes interactions, bacterial genomics, signal transduction
Training of 11 PhD students (7 as main supervisor)
Bibliometry (web of science): 54 peer-reviewed publications H index: 29. External citations: 3541 Average citations per item: 69

Research Areas:

  • Symbiotic nitrogen fixation

Current N-related Projects:


1. Batut J, Terzaghi B, Ghérardi M, Huguet M, Terzaghi E, Garnerone AM, Boistard P et T. Huguet (1985) Localization of a symbiotic fix region on Rhizobium meliloti pSym megaplasmid more than 200 kilobases from the nod-nif region. Mol. Gen. Genet. 199: 232-239

2. Truchet G, Debelle F, Vasse J, Terzaghi B, Garnerone AM, Rosenberg C, Batut J, Maillet F, Denarie J (1985). Identification of a Rhizobium meliloti pSym2011 region controlling the host specificity of root hair curling and nodulation. J Bacteriol. 164:1200-10.

3. Renalier MH, Batut J, Ghai J, Terzaghi B, Gherardi M, David M, Garnerone AM, Vasse J, Truchet G, Huguet T, and Boistard P 1987 A new symbiotic cluster on the pSym megaplasmid of Rhizobium meliloti 2011 carries a functional fix gene repeat and a nod locus. J Bacteriol. 169:2231-8.

4. David M, Daveran ML, Batut J, Dedieu A, Domergue O, Ghai J, Hertig C, Boistard P, Kahn D. (1988) Cascade regulation of nif gene expression in Rhizobium meliloti. Cell. 54:671-83.

5. Cherfils J, Gibrat JF, Levin J, Batut J, Kahn D. (1989) Model-building of Fnr and FixK DNA-binding domains suggests a basis for specific DNA recognition. J Mol Recognit. 2:114-21

6. Batut J, Daveran-Mingot ML, David M, Jacobs J, Garnerone AM, Kahn D. (1989) fixK, a gene homologous with fnr and crp from Escherichia coli, regulates nitrogen fixation genes both positively and negatively in Rhizobium meliloti. EMBO J. 8:1279-86.

7. Hertig C, Li RY, Louarn AM, Garnerone AM, David M, Batut J, Kahn D, Boistard P. (1989) Rhizobium meliloti regulatory gene fixJ activates transcription of R. meliloti nifA and fixK genes in Escherichia coli. J Bacteriol. 171:1736-8.

8. Kahn D, David M, Domergue O, Daveran ML, Ghai J, Hirsch PR, Batut J. (1989) Rhizobium meliloti fixGHI sequence predicts involvement of a specific cation pump in symbiotic nitrogen fixation. J Bacteriol. 171:929-39.

9. de Philip P, Batut J, Boistard P (1990). Rhizobium meliloti FixL is an oxygen sensor and regulates R. meliloti nifA and fixK genes differently in Escherichia coli. J Bacteriol. 172:4255-62.

10. Weiss DS, Batut J, Klose KE, Keener J, Kustu S (1991) The phosphorylated form of the enhancer-binding protein NTRC has an ATPase activity that is essential for activation of transcription. Cell. 67:155-67.

11. Batut J, Santero E, Kustu S (1991). In vitro activity of the nitrogen fixation regulatory protein FIXJ from Rhizobium meliloti. J Bacteriol. 173:5914-7.

12. de Philip, P., Soupène, E., Batut, J. et P. Boistard (1992) Modular structure of the FixL protein of Rhizobium meliloti. Mol. Gen. Genet. 235: 49-54.

13. Waelkens, F., Foglia, A., Morel, J.B., Fourment, J., Batut, J. et P. Boistard (1992) Molecular genetic analysis of the Rhizobium meliloti fixK promoter: identification of sequences involved in positive and negative regulation. Mol. Microbiol. 6: 1447-1456.

14. Reyrat , J.M., David, M., Blonski, C., Boistard, P. et J. Batut (1993) Oxygen-regulated in vitro transcription of Rhizobium meliloti nifA and fixK genes. J. Bacteriol. 175: 6867-6872.

15. Reyrat, J.M., David, M., Batut, J. et P. Boistard (1994) FixL of Rhizobium meliloti enhances the transcriptional activity of a mutant FixJD54N protein by phosphorylation of an alternate residue. J. Bacteriol. 176 : 1969-1976.

16. Galinier, A., Garnerone, A.M., Reyrat, J. M., Kahn, D., Batut, J. et P. Boistard (1994) Phosphorylation of the Rhizobium meliloti FixJ protein induces its binding to a compound regulatory region at the fixK promoter. J. Biol. Chem., 269: 23784-23789.

17. Batut, J. et P. Boistard (1994) Oxygen control in Rhizobium. Antonie van Leeuwenhoek 66: 129-150.

18. Soupène, E., Foussard, M., Boistard, P., Truchet, G., and J. Batut (1995) Oxygen as a key developmental regulator of Rhizobium meliloti N2-fixation gene expression within the alfalfa root nodule. Proc. Natl. Acad. Sci. USA 92: 3759-3763.

19. Foussard M, Garnerone AM, Ni F, Soupène E, Boistard P and J. Batut (1997) Negative autoregulation of the Rhizobium meliloti fixK gene is indirect and requires a newly identified regulator, FixT. Mol. Microbiol. 25: 27-37.

20. Boesten B, Batut J and P. Boistard (1998) DctBD-dependent and -independent expression of the Sinorhizobium (Rhizobium) meliloti C4-dicarboxylate transport gene (dctA) during symbiosis. Mol. Plant-Microbe Interact. 11: 878-886.

21. Garnerone AM, Cabanes D, Foussard M, Boistard and J. Batut (1999) Inhibition of the FixL sensor Kinase by the FixT protein in Sinorhizobium meliloti. J. Biol. Chem., 274: 32500-32506.

22. Barloy-Hubler F, Capela D, Batut J and F. Galibert (2000) High-Resolution Physical Map of the pSymb Megaplasmid and Comparison of the Three Replicons of Sinorhizobium meliloti strain 1021. Current Microbiology (2000): 41:109-13.

23. Cabanes D, Boistard P and J. Batut (2000) Symbiotic Induction of Pyruvate Deydrogenase Genes from Sinorhizobium meliloti. Mol Plant Microbe Interact. 13: 488-493.

24. Cabanes D, Boistard P and J. Batut (2000) Identification of Sinorhizobium meliloti Genes Regulated during Symbiosis. J. Bacteriol. 182: 3632-3637.

25. Bergès H, Checroun C, Guiral S, Garnerone A, Boistard P and J Batut (2001) A glutamine-amidotransferase-like protein modulates FixT anti-kinase activity in Sinorhizobium meliloti. BMC Microbiology 1: 6.

26. Olah B, Kiss E, Gyorgypal Z, Borzi J, Cinege G, Csanadi G, Batut J, Kondorosi A, Dusha I. (2001) Mutation in the ntrR gene, a member of the vap gene family, increases the symbiotic efficiency of Sinorhizobium meliloti. Mol Plant Microbe Interact. 7: 887-894.

27. Capela D, Barloy-Hubler F, Gouzy J, Bothe G, Ampe F, Batut J, Boistard P, Becker A, Boutry M, Cadieu E, Dreano S, Gloux , Godrie T, Goffeau A, Kahn D, Kiss E, Lelaure V, Masuy D, Pohl T, Portetelle D, Puhler A, Purnelle B, Ramsperger U, Renard C, Thebault P, Vandenbol M, Weidner S, Galibert F. 2001. Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti. Proc. Natl. Acad. Sci.USA, 98: 9877-9882.

28. Galibert F, Finan TM, Long SR, Puhler A, Abola P, Ampe F, Barloy-Hubler F, Barnett MJ, Becker A, Boistard P, Bothe G, Boutry M, Bowser L, Buhrmester J, Cadieu E, Capela D, Chain P, Cowie A, Davis RW, Dreano S, Federspiel NA, Fisher RF, Gloux S, Godrie T, Goffeau A, Golding B, Gouzy J, Gurjal M, Hernandez-Lucas I, Hong A, Huizar L, Hyman RW, Jones T, Kahn D, Kahn ML, Kalman S, Keating DH, Kiss E, Komp C, Lelaure V, Masuy D, Palm C, Peck MC, Pohl TM, Portetelle D, Purnelle B, Ramsperger U, Surzycki R, Thebault P, Vandenbol M, Vorholter FJ, Weidner S, Wells DH, Wong K, Yeh KC and Batut J. 2001. The composite genome of the legume symbiont Sinorhizobium meliloti. Science, 293: 668-672.

29. Cosseau C, Garnerone AM and J. Batut (2002) The FixM flavoprotein modulates inhibition by AICAR or 5’AMP of respiratory and nitrogen fixation gene expression in Sinorhizobium meliloti. Mol Plant Microbe Interact. 15: 598-607

30. Bergès, H., Lauber, E., Liebe, C., Batut, J., Kahn, D., de Bruijn, F. and Ampe, F. (2003) Development of Sinorhizobium meliloti macro-arrays for transcriptome analysis. Appl. Environ. Microbiol. 69:1214-1219.

31. Ampe F, Kiss E, Sabourdy F and J. Batut 2003 Transcriptome analysis of Sinorhizobium meliloti genes during symbiosis Genome Biology, 4R15

32. Becker A, Bergès H, Krol E,, Bruand C, Rüberg S, Capela D, Lauber E, Meilhoc E, Ampe F, de Bruijn F, Fourment J, Francez-Charlot A, Kahn D, Küster H, Liebe C, Pühler A, Weidner S, and Batut J (2004) Global changes in gene expression in Sinorhizobium meliloti 1021 under microoxic and symbiotic conditions, Mol Plant Microbe Interact., 17:292-303.

33. Kiss E, Huguet T, Poinsot V, and J. Batut (2004) The typA gene is required for stress adaptation as well as for symbiosis of S. meliloti 1021 with certain M. truncatula lines. Mol Plant Microbe Interact., 17:235-44

34. Cosseau C and J. Batut (2004) Genomics of the ccoNOQP-encoded cbb(3) oxidase complex in bacteria. Arch Microbiol., 181: 89-96 (Revue)

35. Jargeat P., Cosseau C., Ola’h B., Jauneau A., Bonfante P., Batut J. and Bécard G (2004). Isolation, free-living capacities and genome structure of Candidatus Glomeribacter gigasporarum, the endocellular bacterium of the mycorrhizal fungus Gigaspora margarita. J. Bacteriol.,;186:6876-84.

36. Ampe F et J. Batut (2004) Macro-arrays protocols for gene expression studies in bacteria. Molecular Microbial Ecology Manual. Second edition. Kluwer Pub. G. Kowalchuk (ed) sous presse (Chapitre d’ouvrage)

37. Batut J, Andersson SG, O’callaghan D. (2004) The evolution of chronic infection strategies in the alpha-proteobacteria. Nat Rev Microbio l2 (12):933-45.

38. Jamet A, Kiss E, Batut J, Puppo A, Herouart D (2005) The katA catalase gene is regulated by OxyR in both free-living and symbiotic Sinorhizobium meliloti. J Bacteriol. 187(1):376-81.

39. Capela D, Carrère S and Batut J (2005) Transcriptome-based identification of the NodD1 regulon of Sinorhizobium meliloti . Appl. Env. Microbiol. 71(8):4910-3.

40. Capela D, Filipe C, Bobik C, Batut J and Bruand C (2006) . Sinorhizobium meliloti differentiation during symbiosis with alfalfa : a transcriptomic dissection. Mol. Plant Microbe Interact..19:363-372.

41. Bobik C, Meilhoc E and Batut J (2006) FixJ: a major regulator of the oxygen limitation response and late symbiotic functions of Sinorhizobium meliloti. J. Bacteriol.. 188:4890-4902

42. Amadou C, Pascal G, Mangenot S, Glew M, Bontemps C, Capela D, Carrère S, Cruveiller S, Dossat C, Lajus A, Marchetti M, Poinsot V, Rouy Z, Servin B, Saad M, Schenowitz C, Barbe V, Batut J, Médigue C, Masson-Boivin C (2008) Genome sequence of the beta-rhizobium Cupriavidus taiwanensis and comparative genomics of rhizobia. Genome Res. 18(9):1472-83

43. de las Nieves Peltzer M, Roques N, Poinsot V, Aguilar OM, Batut J, Capela D. (2008) Auxotrophy accounts for nodulation defect of most Sinorhizobium meliloti mutants in the branched-chain amino acid biosynthesis pathway. Mol Plant Microbe Interact 21(9):1232-41

44. Becker A, Barnett MJ, Capela D, Dondrup M, Kamp PB, Krol E, Linke B, Rüberg S, Runte K, Schroeder BK, Weidner S, Yurgel SN, Batut J, Long SR, Pühler A, Goesmann A (2009) A portal for rhizobial genomes: RhizoGATE integrates a Sinorhizobium meliloti genome annotation update with postgenome data. J Biotechnol. 40(1-2):45-50.

45. Masson-Boivin C, Giraud E, Perret X, and J. Batut (2009) Establishing nitrogen-fixing symbiosis with legumes: how many rhizobium recipes? Trends in Microbiology, 17 (10):458-466.

46. Marchetti M, Capela D, Glew M, Cruveiller S, Chane-Woon-Ming B, Gris C, Timmers T, Poinsot V, Gilbert L B., Heeb P, Médigue C, Batut J and C. Masson-Boivin. (2010) Experimental evolution of a plant pathogen into a legume symbiont, PloS Biology 8(1):e1000280

47. Marchetti M, Catrice O, Batut J, and C. Masson-Boivin. (2011) Cupriavidus taiwanensis Bacteroids in Mimosa pudica Indeterminate Nodules Are Not Terminally Differentiated Applied and Environmental Microbiology 77 (6): 2161-2164

48. Batut J, Mergeart P and C. Masson-Boivin (2011) Peptide signalling in the rhizobium-legume symbiosis. Current Opinion in Microbiology, 14 (2) 181-187.

49. Tian CF, Garnerone AM, Mathieu-Demazière C, Masson-Boivin C and J. Batut (2012) Plant-activated bacterial receptor adenylate-cyclases modulate epidermal infection in the Sinorhizobium meliloti-Medicago symbiosis. Proc. Natl. Acad. Sci.USA 109(17):6751-6.

50. Marchetti M, Capela D, Poincloux R, Benmeradi N, Auriac M-C, Le Ru A, Maridonneau-Parini I, Batut J, Masson-Boivin C (2013) Queuosine Biosynthesis Is Required for Sinorhizobium meliloti-Induced Cytoskeletal Modifications on HeLa Cells and Symbiosis with Medicago truncatula. PLoS ONE 8(2): e56043. doi:10.1371/journal.pone.0056043

51. Guan SH, Gris C, Cruveiller S, Pouzet C, Tasse L, Leru A, Maillard A, Médigue C, Batut J, Masson-Boivin C, Capela D. (2013) Experimental evolution of nodule intracellular infection in legume symbionts. ISME J. 7:1367-77.

52. Mathieu-Demazière C, Poinsot V, Masson-Boivin C, Garnerone AM and J Batut (2013) Biochemical and functional characterization of SpdA, a 2′, 3′Cyclic Nucleotide Phosphodiesterase from Sinorhizobium meliloti. BMC Microbiology, 13:268.

53. Remigi P, Capela D, Clerissi C, Tasse L, Torchet R, Bouchez O, Batut J, Cruveiller S, Rocha EP, Masson-Boivin C (2014) Transient Hypermutagenesis Accelerates the Evolution of Legume Endosymbionts following Horizontal Gene Transfer. PLoS Biol.12(9):e1001942.

54. Marchetti M, Jauneau A, Capela D, Remigi P, Gris C, Batut J, Masson-Boivin C.(2014) Shaping bacterial symbiosis with legumes by experimental evolution. Mol Plant Microbe Interact. 27(9):956-64