Profile – Esperanza Martínez-Romero

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Esperanza Martínez-Romero

Job Title:

Professor and researcher at the Genomic Science Center, National University of Mexico, Cuernavaca


Phone Number:


Google Scholar Profile


B.S. (Cum Laude), Universidad Nacional Autónoma de México (UNAM) 1981.
M.S. (Cum Laude), UNAM 1983.
Ph.D. (Cum Laude), UNAM 1885.
Postdoctoral position in the Lab. de Biologie Moleculaire des Plantes-Microorganismes, Dr. Jean Dénarié, Toulouse, France, 1985.

Recent or forthcoming activities

– Invited as plenary speaker opening session at the Microbe-assisted crop production – opportunities, challenges and needs” (miCROPe 2017). Vienna Austria, December 2017
– Organizer of III IBEMPA – XXVIII RELAR – XVI SEFIN. Lima Peru, November 2017
– Invited speaker at the 20th International Congress on Nitrogen Fixation. Granada Spain, September 2017
– Invited as plenary speaker opening session at the International Symposium on Functional Genomics and System Biology. Cuernavaca Mexico, May 2017
– Invited reviewer of a Ph D. thesis from Murdoch Australia, February 2017.
– Invited as associate editor of Genome Biology and Evolution Journal. Nov. 2016
– Invited speaker at the XXXI National Biochemistry Congress, Aguascalientes Mexico, November 2016.
– Invited speaker at the XXVII Reunión Latinoamericana de Rizobiologia. Londrina Brazil, June 2016.
– Organizer of MicrobiomeX Symposium, Mexico City, May 2016.
– 2nd position of priority among around 400 approved grants (1/5 approved of all submissions) to get financial Basic Science support from CONACyT. 2016
– Speaker at the 23rd North American Conference on Symbiotic Nitrogen Fixation, Ixtapa Mexico. December 2015.
– Coordinator since July 2013 of the Undergraduate Program on Genomic Science, a very successful academic program with an academic level comparable to highly recognized universities in the US and Europe.

Prize of Science from the Mexican Academy of Sciences 1996.
Award to Young Scientists from the National University of Mexico 1996.
Award from the National Research Council as “The Best Student of Mexico”, 1981.
Biography included in Who is Who in Science and Engineering, 1998-1999.
Prize from the National University of Mexico, Juana Ramírez de Asbaje for outstanding Mexican women scientists, 2003.
Award from ISI for two most cited Mexican papers, 1999.
Prize National University of Mexico, 2005.
Woman of the Year for Mexico by the American Biogeographical Institute 2008.
Distinction as Fellow of the American Academy of Microbiology, January 2009.
Elected member of the board of directors of the ISME in 2011.
AgroBIO research- career award 2011.
SCOPUS recognition (2013) as one of the most cited Mexican Scientists in 2012
REMEI prize. Morelos State Prize to Outstanding Scientists from the Secretary of Science, Innovation and Technology, November 27, 2014

Citations. From Google Scholar H=63. iH10=152, citations over 12 200

International activities

Member of the Board of Directors of the International Society for Microbial Ecology, ISME
Selected as training laboratory for Latin America for the Biofertilization Web BIOFAG.
Ambassador of ASM (American Society for Microbiology) and ISME (International Society Microbial Ecology) in Mexico. International Steering Committee of the International Congress on Nitrogen Fixation, Pacific Grove,California, October 4-9, 2015.
Referee of promotion of a Professor at the University of Florida, August 2014.
Referee of Genome Canada Grants, October 2014

Applied and Environmental Microbiology 2011-2012
Genome Biology and Evolution since dic 21016 (associate editor).

Member of Editorial Boards:
Archives of Microbiology 1998-2000, Applied and Environmental Microbiology 2001-, Molecular Plant-Microbe Interactions 2004-2006, Journal of Bacteriology 2006-2010, Microbial Ecology 2006, ISME Journal 2007-2008, DNA and Cell Biology 2008-2011, Systematic and Applied Microbiology 2011-

Book editor:
Highlights of Nitrogen Fixation Research (eds.) Kluwer Academic / Plenum Publishers, New York, 1999.
Microbes on Line UNAM, 2001 and 2004, México.

President of the International Taxonomy Committee Sc. Rhizobium and Agrobacterium since January 1996, of the National Society of Biological Nitrogen Fixation, 1989-1991. President of the Mexican Society for Microbiology, 2004-2006, vice-president, 2002-2004

Organizing committees of international and national congresses on Nitrogen Fixation, Microbial Ecology, Microbial biodiversity and others since 1998.

Reviewer of international grants and Reviewer of Ph.D theses from the University of Helsinki in Finland, The Catholic State University, Leuven, Belgium and the University W. Australia.

Member of the evaluation committee (groups and young scientist proposals) from the Mexican National Research Council (CONACyT) since 2014 and of the UNAM grant program PAPIIT 2013-2016.

Reviewer of articles for international journals:
Antonie van Leuwenhoek, Australian Journal of Experimental Agriculture, Biotrópica, Canadian Journal of Microbiology, Critical Reviews in Biotechnology, FEMS Microbiology Letters, Genome Biology and Evolution, International Journal of Systematic Bacteriology, International of Systematic and Evolutionary Microbiology, Journal of Applied Bacteriology, Journal of Biotechnology, Journal of Basic Microbiology, Microbiology, Microbial Ecology, Molecular Microbiology, Molecular Ecology, Plant-Microbe Interactions, Plant and Soil, Research in Microbiology, Soil Biology and Biochemistry, Symbiosis, PLoS One, Frontiers in Plant Sciences

Responsible for the Ribotypification Unit that provided service for the identification of bacteria based on the sequence of 16S ribosomal genes (1998-2004).

from GEF-CIAT 2003-2009, VLIR Belgium, FAO, Agropolis (France), EU, UNAM, National Research Council, CONACyT (Mexico)

Sabbatical years
UC Davis, California, Sept. 1998 – August 1999
UC Davis, California, August 2006-August 2007.

Visiting scientist
Bielefeld University, Germany; Paul Sabatier University, Toulouse, France; EMBRAPA, Brazil;
Uppsala University, Sweden.

Social contributions
-Rhizobial inoculant production for farmers in distinct regions in Mexico. In the rain forest of Los Tuxtlas the aim is to preserve the forest by maintaining soil fertility , thus new forests do not need to be tilled. Ongoing programs.
-Reforestation with legumes and rhizobial inoculants in Morelos.
-Talks to farmers and children on biofertilization.
-Microbial evaluation of polluted rivers in Morelos that led to political decisions to build sewage treatment plants in Cuernavaca. Member of the ravine council of Morelos 2003-2007.
-Preservation of local legume and Jatropha germplasm (native plants species that is used for biodiesel production) and identification of toxic and non toxic Jatropha cultivars.
Editor and author of a Manual for farmers on Biofertilizers, September 2013.
TV and radio interviews

Teaching duties:
Graduate and undergraduate courses in Microbiology, Microbial Ecology, Molecular Biology of Symbiotic Interactions and Metagenomics, Applications of Genomics, Prokaryotic Genomics, Logical Analysis of Bacterial Genomic Articles and Lectures
22 students from abroad and 18 from other institutions in Mexico have received training in Dr. Martínez-Romero’s lab.
Ph. D. theses concluded as direct tutor = 22

Research Areas:

  • Plant-microbe interactions
  • Rhizobia-legume symbiosis
  • Rhizobia
  • Biological nitrogen fixation
  • Genomics

Current N-related Projects:



Santamaría RI, Bustos P, Pérez-Carrascal OM, Miranda-Sánchez F, Vinuesa P, Martínez-Flores I, Juárez S, Lozano L, Martínez-Romero E, Cevallos MÁ, Romero D, Dávila G, Ormeño-Orrillo E, González V. 2017. Complete genome sequences of eight Rhizobium symbionts associated with common bean (Phaseolus vulgaris). Genome Announc 5:e00645-17.

Ormeño-Orrillo E, Rey L, Durán D, Canchaya CA, Rogel MA, Zúñiga-Dávila D, Imperial J, Ruiz-Argüeso T, Martínez-Romero E. Draft genome sequence of Bradyrhizobium paxllaeri LMTR 21T isolated from Lima bean (Phaseolus lunatus) in Peru. Genomics Data. 2017 13:38-40. doi: 10.1016/j.gdata.2017.06.008.

Ormeño-Orrillo E, Rey L, Durán D, Canchaya CA, Zúñiga-Dávila D, Imperial J, Martínez-Romero E, Ruiz-Argüeso T. Genome sequence of Bradyrhizobium sp. LMTR 3, a diazotrophic symbiont of Lima bean (Phaseolus lunatus). Genomics Data. 2017 13:35-37. doi: 10.1016/j.gdata.2017.06.007.
Bustos P, Santamaría RI, Pérez-Carrascal OM, Acosta JL, Lozano L, Juárez S, Martínez-Flores I, Martínez-Romero E, Cevallos MÁ, Romero D, Dávila G, Vinuesa P, Miranda F, Ormeño E, González V. Complete genome sequences of three Rhizobium gallicum symbionts associated with common bean (Phaseolus vulgaris). Genome Announcements. 2017 Mar 16;5(11). doi: 10.1128/genomeA.00030-17.

Gamez-Reyes A, Becerra-Lobato N, Ramirez-Trujillo JA, Martinez-Romero E, Dunn MF, Hernandez-Lucas I. 2017. The Rhizobium leucaenae CFN 299 pSym plasmid contains genes expressed in free life and symbiosis, as well as two replication systems Annals of Microbiology. 67:263-273.

Torres Tejerizo G, Wibberg D, Winkler A, Ormeño-Orrillo E, Martínez-Romero E, Niehaus K, Pühler A, Kalinowski J, Lagares A, Schlüter A, Pistorio M. Genome sequence of the symbiotic type strain Rhizobium tibeticum CCBAU85039T. Genome Announcements. 2017 Jan 26;5(4). pii: e01513-16. doi: 10.1128/genomeA.01513-16.

de Lajudie P, Martinez-Romero E. International Committee on Systematics of Prokaryotes Subcommittee on the taxonomy of Agrobacterium and Rhizobium Minutes of the meeting, 7 September 2014, Tenerife, Spain. Int J Syst Evol Microbiol. 2017 Feb;67(2):516-520. doi: 10.1099/ijsem.0.001597.


Peralta H, Aguilar A, Díaz R, Mora Y, Martínez-Batallar G, Salazar E, Vargas-Lagunas C, Martínez E, Encarnación S, Girard L, Mora J. Genomic studies of nitrogen-fixing rhizobial strains from Phaseolus vulgaris seeds and nodules. BMC Genomics. 2016 Sep 6;17:711. doi: 10.1186/s12864-016-3053-z.

Degli Esposti M, Martinez Romero E.  A survey of the energy metabolism of nodulating symbionts reveals a new form of respiratory complex I.
FEMS Microbiol Ecol. 2016 Jun;92(6). pii: fiw084. doi: 10.1093/femsec/fiw084.

Torres Tejerizo G, Rogel MA, Ormeño-Orrillo E, Althabegoiti MJ, Nilsson JF, Niehaus K, Schlüter A, Pühler A, Del Papa MF, Lagares A, Martínez-Romero E, Pistorio M. Rhizobium favelukesii sp. nov., isolated from the root nodules of alfalfa (Medicago sativa L). Int J Syst Evol Microbiol. 2016 Aug 3. doi: 10.1099/ijsem.0.001373.

Servín-Garcidueñas LE, Rogel MA, Ormeño-Orrillo E, Zayas-Del Moral A, Sánchez F, Martínez-Romero E. Complete Genome Sequence of Bradyrhizobium sp. Strain CCGE-LA001, Isolated from Field Nodules of the Enigmatic Wild Bean Phaseolus microcarpus. Genome Announc. 2016 Mar 17;4(2). pii: e00126-16. doi: 10.1128/genomeA.00126-16.

Bontemps C, Rogel MA, Wiechmann A, Mussabekova A, Moody S, Simon MF, Moulin L, Elliott GN, Lacercat-Didier L, Dasilva C, Grether R, Camargo-Ricalde SL, Chen W, Sprent JI, Martínez-Romero E, Young JP, James EK. Endemic Mimosa species from Mexico prefer alphaproteobacterial rhizobial symbionts. New Phytol. 2016 Jan;209(1):319-33. doi: 10.1111/nph.13573.


Delamuta JR, Ribeiro RA, Ormeño-Orrillo E, Parma MM, Melo IS, Martínez-Romero E, Hungria M. Bradyrhizobium tropiciagri sp. nov. and Bradyrhizobium embrapense sp. nov., nitrogen-fixing symbionts of tropical forage legumes. Int J Syst Evol Microbiol. 2015 Dec;65(12):4424-33. doi: 10.1099/ijsem.0.000592.

Helene LC, Delamuta JR, Ribeiro RA, Ormeño-Orrillo E, Rogel MA, Martínez-Romero E, Hungria M. Bradyrhizobium viridifuturi sp. nov., encompassing nitrogen-fixing symbionts of legumes used for green manure and environmental services. Int J Syst Evol Microbiol. 2015 Dec;65(12):4441-8. doi: 10.1099/ijsem.0.000591.

Ribeiro RA, Martins TB, Ormeño-Orrillo E, Marçon Delamuta JR, Rogel MA, Martínez-Romero E, Hungria M. Rhizobium ecuadorense sp. nov., an indigenous N2-fixing symbiont of the Ecuadorian common bean (Phaseolus vulgaris L.) genetic pool. Int J Syst Evol Microbiol. 2015 Sep;65(9):3162-9. doi: 10.1099/ijsem.0.000392.

Ormeño-Orrillo E, Servín-Garcidueñas LE, Rogel MA, González V, Peralta H, Mora J, Martínez-Romero J, Martínez-Romero E. Taxonomy of rhizobia and agrobacteria from the Rhizobiaceae family in light of genomics. Systematic and Applied Microbiology. 2015 Jun;38(4):287-91. doi: 10.1016/j.syapm.2014.12.002


Rogel MA, Bustos P, Santamaría RI, González V, Romero D, Cevallos MÁ, Lozano L, Castro-Mondragón J, Martínez-Romero J, Ormeño-Orrillo E, Martínez-Romero E. Genomic basis of symbiovar mimosae in Rhizobium etli. BMC Genomics. 2014. 15:575. doi: 10.1186/1471-2164-15-575.

Dall’Agnol RF, Ribeiro RA, Delamuta JR, Ormeño-Orrillo E, Rogel MA, Andrade DS, Martínez-Romero E, Hungria M. Rhizobium paranaense sp. nov., an effective N2-fixing symbiont of common bean (Phaseolus vulgaris L.) with broad geographical distribution in Brazil. Int J Syst Evol Microbiol. 2014. 64(Pt 9):3222-9. doi: 10.1099/ijs.0.064543-0.

Servín-Garcidueñas LE, Zayas-Del Moral A, Ormeño-Orrillo E, Rogel MA, Delgado-Salinas A, Sánchez F, Martínez-Romero E. Symbiont shift towards Rhizobium nodulation in a group of phylogenetically related Phaseolus species. Mol Phylogenet Evol. 2014. 79:1-11. doi: 10.1016/j.ympev.2014.06.006.

Siqueira AF, Ormeño-Orrillo E, Souza RC, Rodrigues EP, Almeida LG, Barcellos FG, Batista JS, Nakatani AS, Martínez-Romero E, Vasconcelos AT, Hungria M. Comparative genomics of Bradyrhizobium japonicum CPAC 15 and Bradyrhizobium diazoefficiens CPAC 7: elite model strains for understanding symbiotic performance with soybean. BMC Genomics. 2014. 15:420. doi: 10.1186/1471-2164-15-420.

Durán D, Rey L, Mayo J, Zúñiga-Dávila D, Imperial J, Ruiz-Argüeso T, Martínez-Romero E, Ormeño-Orrillo E. Bradyrhizobium paxllaeri sp. nov. and Bradyrhizobium icense sp. nov., nitrogen-fixing rhizobial symbionts of Lima bean (Phaseolus lunatus L.) in Peru. Int J Syst Evol Microbiol. 2014. 64(Pt 6):2072-8. doi: 10.1099/ijs.0.060426-0.

Althabegoiti MJ, Ormeño-Orrillo E, Lozano L, Torres Tejerizo G, Rogel MA, Mora J, Martínez-Romero E. Characterization of Rhizobium grahamii extrachromosomal replicons and their transfer among rhizobia. BMC Microbiol. 2014. 14:6. doi: 10.1186/1471-2180-14-6.


Aline López-López, Simoneta Negrete-Yankelevich, Marco A. Rogel, Ernesto Ormeño-Orrillo, Julio Martínez, Esperanza Martínez-Romero. Native bradyrhizobia from Los Tuxtlas in Mexico are symbionts of Phaseolus lunatus (Lima bean). Systematic and Applied Microbiology 36:33-38 (2013). IS – 1618-0984 doi: 10.1016/j.syapm.2012.10.006

Ernesto Ormeño-Orrillo, Esperanza Martínez-Romero. Phenotypic tests in Rhizobium species description: An opinión and (a sympatric speciation) hypothesis. Systematic and Applied Microbiology 36:145-147 (2013). IS – 1618-0984, doi:10.1016/j.syapm.2012.11.009

Ernesto Ormeño-Orrillo, Luis E. Servín-Garcidueñas, Juan Imperial, Luis Rey, Tomás Ruiz-Argueso, Esperanza Martínez-Romero. Phylogenetic evidence of the transfer of nodZ and nodL genes from Bradyrhizobium. Molecular Phylogenetics and Evolution 67: 626-630 (2013). IS – 1095-9513, doi: 10.1016/j.ympev.2013.03.003

Jesús Morales-Jiménez, Arturo Vera-Ponce de León, Aidé García-Domínguez, Esperanza Martínez-Romero, Gerardo Zúñiga, César Hernández-Rodríguez. Nitrogen-fixing and uricolytic bacteria associated with the gut of Dendroctonus rhizophagus and Dendroctonus valens (Curculionidae: Scotytinae). Microbial Ecology 66: 200-210 (2013). IS- 1432-184X, doi: 10.10007/s00248-013-0206-3

Jakeline Renata Marçon Delamuta, Renan Augusto Ribeiro, Ernesto Ormeño-Orrillo, Itamar Soares Melo, Esperanza Martínez-Romero, Mariangela Hungria. Polyphasic evidence supporting the reclassification of Bradyrhizobium japonicum Group Ia strains as Bradyrhizobium diazoefficiens sp. nov. International Journal of Systematic and Evolutionary Microbiology 63, 3342–3351 (2013). Factor de Impacto 2.112, PUBMED, ISSN – 1466-5034, doi:10.1099/ijs.0.049130-0

Renan Augusto Ribeiro, Ernesto Ormeño-Orrillo, Rebeca Fuzinatto Dall’Agnol, Peter H. Graham, Esperanza Martinez-Romero, Mariangela Hungria. Novel Rhizobium lineages isolated from root nodules of the common bean (Phaseolus vulgaris L.) in Andean and Mesoamerican areas. Research in Microbiology 164 (7):740-7488 (2013). ISSN – 1769-7123, doi: 10.1016/j.resmic.2013.05.002.

Reiner Rincón-Rosales, José M. Villalobos-Escobedo, Marco A. Rogel, Aline López-López, Julio Martinez, Ernesto Ormeño- Orrillo, Esperanza Martínez-Romero. Rhizobium calliandrae, Rhizobium mayense and Rhizobium jaguaris, novel rhizobial species nodulating the medicinal legume Calliandra grandiflora. International Journal of Systematic and Evolutionary Microbiology 63: 3423–3429 (2013). ISSN – 1466-5034, doi 10.1099/ijs.0.048249-0.

Rebeca Fuzinatto Dall’Agnol, Renan Augusto Ribeiro, Ernesto Ormeño-Orrillo, Marco Antonio Rogel, Jakeline Renata Marçon Delamuta, Diva Souza Andrade, Esperanza Martínez-Romero,
Mariangela Hungria. Rhizobium freirei, a symbiont of Phaseolus vulgaris very effective in fixing nitrogen. International Journal of Systematic and Evolutionary Microbiology 63 (11): 4167–4173. ISSN – 1466-5034, 2013. doi:10.1099/ijs.0.052928-0


Ernesto Ormeño-Orrillo, Pamela Menna, Luis Gonzaga P Almeida, Francisco Javier Ollero, Marisa Fabiana Nicolás, Elisete Pains Rodrigues, Andre Shigueyoshi Nakatani, Jesiane Stefania Silva Batista, Ligia Maria Oliveira Chueire, Rangel Celso Souza, Ana Tereza Ribeiro Vasconcelos, Manuel Megías, Mariangela Hungria and Esperanza Martínez-Romero. Genomic basis of broad host range and environmental adaptability of Rhizobium tropici CIAT 899 and Rhizobium sp. PRF 81 which are used in inoculants for common bean (Phaseolus vulgaris.) BMC Genomics 13:735.

Ernesto Ormeño-Orrillo, Marco A. Rogel, Ligia Maria Oliveira Chueire, James M. Tiedje, Esperanza Martínez-Romero, and Mariangela Hungria. Genome sequences of Burkholderia sp. strains CCGE1002 and H160, isolated from legume nodules in Mexico and Brazil. Journal of Bacteriology 194:6927.

M. Julia Althabegoiti, Luis Lozano, Gonzalo Torres Tejerizo, Ernesto Ormeño-Orrillo, Marco. A Rogel, Víctor González, Esperanza Martínez-Romero. Genome of Rhizobium grahamii CCGE 502, a broad-host-range symbiont with low nodulation competitiveness in Phaseolus vulgaris. Journal of Bacteriology 194:6651-6652.

Yendi E. Navarro-Noya, Emma Hernández-Mendoza, Jesús Morales-Jiménez, Janet Jan-Roblero, Esperanza Martínez-Romero, César Hernández-Rodríguez. Isolation and characterization of nitrogen fixing heterotrophic bacteria from the rhizosphere of pioneer plants growing on mine tailings. Applied Soil Ecology 62:52-60.

Servin-Garciduenas, L.E., Rogel, M.A., Ormeno-Orrillo, E., Delgado-Salinas, A., Martínez-Romero, J., Sánchez, F. and Martínez-Romero, E. Genome sequence of Rhizobium sp. strain CCGE510, a symbiont isolated from nodules of the endangered wild bean Phaseolus albescens. Journal of Bacteriology 194:6310-6311 (2012).

Ernesto Ormeño-Orrillo, Marco A. Rogel-Hernández, Lourdes Lloret, Aline López-López, Julio Martínez, Isabelle Barois, Esperanza Martínez-Romero. Change in land use alters the diversity and composition of Bradyrhizobium communities and led to the introduction of Rhizobium etli into the tropical rain forest of Los Tuxtlas (Mexico). Microbial Ecology 63:822-834.

López-López, A., M. A. Rogel-Hernández, I. Barois, A. I. Ortiz Ceballos, J. Martínez, E. Ormeño-Orrillo, E. Martinez-Romero. Rhizobium grahamii sp. nov. from Dalea leporina, Leucaena leucocephala, Clitoria ternatea nodules, and Rhizobium mesoamericanum sp. nov. from Phaseolus vulgaris, siratro, cowpea and Mimosa pudica nodules. International Journal of Systematic and Evolutionary Microbiology 62:2264-2271 (2012).

Doreen Fischer, Barbara Pfitzner, Michael Schmid,, Jean L. Simoes-Araujo, Veronica M. Reis, William Pereira, Ernesto Ormeño-Orrillo, Brigitte Hai, Andreas Hofmann, Michael Schloter, Esperanza Martínez-Romero, Jose Ivo Baldani, Anton Hartmann. Molecular characterisation of the diazotrophic bacterial community in uninoculated and inoculated field-grown sugarcane (Saccharum sp.). Plant and Soil 356:83-99.


Marco A. Rogel, Ernesto Ormeño-Orrillo, Esperanza Martínez Romero. Symbiovars in rhizobia reflect bacterial adaptation to legumes. Systematic and Applied Microbiology 34:96-104 (2011).

Aline López-López, Marco A. Rogel, Ernesto Ormeño-Orrillo, Julio Martínez-Romero, Esperanza Martínez-Romero. Phaseolus vulgaris seed-borne endophytic community with novel bacterial species such as Rhizobium endophyticum. Systematic and Applied Microbiology 33(6):322-327


Reiner Rincón-Rosales, Lourdes Lloret, Edith Ponce and Esperanza Martínez- Romero. Rhizobia with different symbiotic efficiencies nodulate Acaciella angustissima in Mexico, including Sinorhizobium chiapanecum sp. nov. which has common symbiotic genes with Sinorhizobium mexicanum. FEMS Microbiology Ecology 67:103-117

Geoffrey N. Elliott, Jui-Hsing Chou, Wen-Ming Chen, Guido V. Bloemberg, Cyril Bontemps, Esperanza Martínez-Romero, Encarna Velázquez, J. Peter W. Young, Janet I. Sprent and Euan K. James. Burkholderia spp. are the most competitive symbionts of Mimosa, particularly under N-limited conditions. Environmental Microbiology 11:762-778

Esperanza Martínez-Romero. Coevolution in Rhizobium-Legume Symbiosis? DNA and Cell Biology 28:361-370.


Lourdes Lloret, Ernesto Ormeño-Orrillo, Reiner Rincón, Julio Martínez-Romero, Marco Antonio Rogel-Hernández, Esperanza Martínez-Romero. Ensifer mexicanus sp. nov. a new species nodulating Acacia angustissima (Mill.) Kuntze in Mexico. Systematic and Applied Microbiology 30:280-290

Roseline Remans, Carla Snoeck, Christel Verte, Anja Croonenborghs, Ellen Luyten, Maxime Ndayizeye, Esperanza Martínez-Romero, Jan Michiels and Jos Vanderleyden. Inactivation of the nodH gene in Sinorhizobium sp. BR816 enhances symbiosis with Phaseolus vulgaris L. FEMS Microbiology Letters 266:210-217

Claudia Silva, Feng Ling Kan and Esperanza Martínez-Romero. Population genetic structure of Sinorhizobium meliloti and S. medicae isolated from nodules of Medicago spp. in Mexico. FEMS Microbiology Ecology 60:477-489

Luciana Grange, Mariangela Hungria, Peter H. Graham, Esperanza Martínez-Romero. New insights into the origins and evolution of rhizobia that nodulate common bean (Phaseolus vulgaris) in Brazil. Soil Biology and Biochemistry 39:867-876.


En Tao Wang, Zhi Yuan Tan, Xian Wu Guo, Rolando Rodríguez-Durán, Gisela Boll, Esperanza Martínez-Romero. Diverse endophytic bacteria isolated from a leguminous tree Conzattia multiflora grown in Mexico. Archives of Microbiology 186:251-259.

Mónica Rosenblueth and Esperanza Martínez-Romero. Bacterial endophytes and their interactions with hosts. Molecular Plant-Microbe Interactions 19:827-837.

Ismael Hernández-Lucas, José Augusto Ramírez-Trujillo, Miguel Angel Gaitan, Xianwu Guo, Margarita Flores, Esperanza Martínez-Romero, Ernesto Pérez-Rueda & P. Mavingui. Isolation and characterization of functional insertion sequences of rhizobia. FEMS Microbiology Letters 261:25-31.

Pâmela Menna, Mariangela Hungria, Fernando G. Barcillos, Eliane V. Bangel, Pablo N. Hess, Esperanza Martínez-Romero. Molecular phylogeny based on the 16S rRNA gene of elite rhizobial strains used in Brazilian commercial inoculants. Systematic and Applied Microbiology 29:315-332.

Fátima Maria de Souza Moreira, Leonardo Cruz, Sérgio Miana de Faria, Terence Marsh, Esperanza Martínez-Romero, Fábio de Oliveira Pedrosa, Rosa Maria Pitard and J. Peter W. Young.
Azorhizobium doebereinerae sp. nov. microsymbiont of Sesbania virgata (Caz.) Pers. Systematic and Applied Microbiology 29:197-206.

Ernesto Ormeño-Orillo, Pablo Vinuesa, Doris Zúñiga-Dávila, Esperanza Martínez-Romero. Molecular diversity of native bradyrhizobia isolated from Lima bean (Phaseolus lunatus L.) in Peru.
Systematic and Applied Microbiology 29:253-262.


P. Vinuesa, C. Silva, M.J. Lorite, M.L. Izaguirre-Mayoral, E.J. Bedmar, E. Martínez-Romero. Molecular systematics of rhizobia based on maximum likelihood and Bayesian phylogenies inferred from rrs, atpD, recA and nifH sequences, and their use in the classification of Sesbania mycrosymbionts from Venezuelan wetlands. Systematic and Applied Microbiology 28:702-716.

Claudia Silva, Pablo Vinuesa, Luis E. Eguiarte, Valeria Souza, and Esperanza Martínez-Romero. Evolutionary genetics and biogeographic structure of Rhizobium gallicum sensu lato, a widely distributed bacterial symbiont of diverse legumes. Molecular Ecology 14:4033-4050.

P. Vinuesa, M. León-Barrios, C. Silva, A. Willems, A. Jarabo-Lorenzo, R. Pérez-Galdona, D. Werner and E. Martínez-Romero. Bradyrhizobium canariense sp. nov., an acid-tolerant endosymbiont that nodulates endemic genistoid legumes (Papilionoideae: Genisteae) from the Canary Islands, along with Bradyrhizobium japonicum bv. genistearum, Bradhyrhizobium genoespecies α and Bradyrhizobium genoespecies β. International Journal of Systematic and Evolutionary Microbiology 55:569-575


J.M. Young, L.D. Kuykendall, E. Martínez-Romero, A. Kerr and H. Sawada. Classification and nomenclature of Agrobacterium and Rhizobium – a reply to Farrand et al. (2003). International Journal of Systematic and Evolutionary Microbiology 53:1689-1695.

Lucía Martínez, Jesús Caballero, José Orozco and Esperanza Martínez-Romero. Diazotrophic bacteria associated with banana (Musa spp.). Plant and Soil 257:35-47.

Eduardo Valencia-Cantero, Juan José Peña-Cabriales, Esperanza Martínez-Romero. The corrosion effects of sulfate- and ferric-reducing bacterial consortia on steel. Geomicrobiology Journal 20:157-169.

Anne Willems, Manuel Fernández-López, Estefanía Muñoz-Adelantado, Johan Goris, Paul De Vos, Esperanza Martínez-Romero, Nicolás Toro, Monique Gillis. Description of New Ensifer strains from nodules and proposal to transfer Ensifer adhaerens Casida 1982 to Sinorhizobium as Sinorhizobium adhaerens comb. nov. request for an opinion. International Journal of Systematic and Evolutionary Microbiology 53:1207-1217.

Esperanza Martínez-Romero. Diversity of Rhizobium-Phaseolus vulgaris symbiosis: overview and perspectives. Plant and Soil 252:11-23.

Carla Snoeck, Christel Verreth, Ismael Hernández-Lucas, Esperanza Martínez-Romero, and Jos Vanderleyden. Identification of a third sulfate activation system in Sinorhizobium sp. strain BR816: the CysDN sulfate activation complex. Applied and Environmental Microbiology 69:2006-2014.

Ivonne Toledo, Lourdes Lloret, and Esperanza Martínez-Romero. Sinorhizobium americanum sp. nov., a new Sinorhizobium species nodulating native Acacia spp. in Mexico.
Systematic and Applied Microbiology 26:54-64.

C. Silva, Pablo Vinuesa, Luis E. Eguiarte, Esperanza Martínez-Romero and Valeria Sousa. Rhizobium etli and Rhizobium gallicum nodulate common bean (Phaseolus vulgaris) in a traditionally managed milpa plot in Mexico: population genetics and biogeographic implications. Applied and Environmental Microbiology 69:884-893


I. Hernández-Lucas, P. Mavingui, T. Finan, P. Chain and E. Martínez-Romero. In vivo cloning strategy for Rhizobium plasmids. BioTechniques 33:1-4.

Carlos Acosta-Durán, Esperanza Martínez-Romero. Diversity of rhizobia from nodules of the leguminous tree Gliricidia sepium, a natural host of Rhizobium tropici. Archives of Microbiology 178:161-164.

En Tao Wang, M. Antonio Rogel, Xin Hua Sui, Wen Xin Chen, Esperanza Martínez-Romero, Peter van Berkum. Mesorhizobium amorphae, a rhizobial species that nodulates Amorpha fructicosa, is native to American soils. Archives of Microbiology 178:301-305.

A. Sessitsch, J.G. Howieson, X. Perret, H. Antoun, and E. Martínez-Romero. Advances in Rhizobium Research. Critical Reviews in Plant Sciences 21:323-378.


M.L. Gutiérrez-Zamora and E. Martínez-Romero. Natural endophytic association between Rhizobium etli and maize (Zea mays L.). Journal of Biotechnology 91:117-126.

L.E. Fuentes-Ramírez, R. Bustillos-Cristales, A. Tapia-Hernández, T. Jiménez-Salgado, E.T. Wang, E. Martínez-Romero and J. Caballero-Mellado. Novel nitrogen-fixing acetic acid bacteria, Gluconacetobacter johannae sp. nov. and Gluconacetobacter azotocaptans sp. nov., associated with coffee plants. International Journal of Systematic and Evolutionary Microbiology 51:1305-1314.

M.A. Rogel, Ismael Hernández-Lucas, L.D. Kuykendall, D.L. Balkwill and E. Martínez-Romero. Nitrogen-fixing nodules with Ensifer adhaerens harboring Rhizobium tropici symbiotic plasmids.
Applied and Environmental Microbiology 67:3264-3268.


Adriana Jarabo-Lorenzo, Encarna Velázquez, Ricardo Pérez-Galdona, María C. Vega-Hernández, Eustoquio Martínez-Molina, Pedro F. Mateos, Pablo Vinuesa, Esperanza Martínez-Romero and Milagros León-Barrios. Restriction fragment length polymorphism analysis of 16S rDNA and low molecular weight RNA profiling of rhizobial isolates from shrubby legumes endemic to the Canary Islands. Systematic and Applied Microbiology 23:418-425.

E.T. Wang and E. Martínez-Romero. Sesbania herbacea-Rhizobium huautlense nodulation in flooded soils and comparative characterization of S. herbacea-nodulating rhizobia in different environments. Microbial Ecology 40:25-32.

Adama Diouf, Philippe de Lajudie, Marc Neyra, Karel Kersters, Monique Gillis, Esperanza Martínez-Romero and Mamadou Gueye. Polyphasic characterization of rhizobia that nodulate Phaseolus vulgaris in west Africa (Senegal and Gambia). International Journal Systematic Bacteriology 50:159-170.


J.A. Herrera-Cervera, J. Caballero-Mellado, G. Laguerre, H.V. Tichy, N. Requena, N. Amarger, E. Martínez-Romero, J. Olivares, J. Sanjuan. At least five rhizobial species nodulate Phaseolus vulgaris in a Spanish soil. FEMS Microbiology Ecology 30:87-97.

T. Laeremans, C. Snoeck, J. Mariën, C. Verreth, E. Martínez-Romero, J.-C. Promé and J. Vanderleyden. Phaseolus vulgaris recognizes Azorhizobium caulinodans Nod factors with a variety of chemical substituents. Molecular Plant-Microbe Interactions 12:820-824.

Z.Y. Tan, E.T. Wang, G.X. Peng, M.E. Zhu, E. Martínez-Romero and W.X. Chen. Characterization of bacteria isolated from wild legumes in the Northwestern regions of China. International Journal of Systematic Bacteriology 49:1457-1469.

En Tao Wang, M.A. Rogel, Alejandro García-de los Santos, Julio Martínez-Romero, Miguel A. Cevallos and Esperanza Martínez-Romero. Rhizobium etli bv. mimosae, a novel biovar isolated from Mimosa affinis. International Journal of Systematic Bacteriology 49:1479-1491.

Marcela Castillo, Margarita Flores, Patrick Mavingui, Esperanza Martínez-Romero, Rafael Palacios and Georgina Hernández. Increase in alfalfa nodulation, nitrogen fixation and plant growth by specific DNA amplification in Sinorhizobium meliloti. Applied and Environmental Microbiology 65:2716-2722.

L.E. Fuentes-Ramírez, J. Caballero-Mellado, J. Sepúlveda and E. Martínez-Romero. Colonization of sugarcane by Acetobacter diazotrophicus is inhibited by high N-fertilization. FEMS Microbiology Ecology 29:117-128.

En Tao Wang, Julio Martínez-Romero and Esperanza Martínez-Romero. Genetic diversity of rhizobia from Leucaena leucocephala nodules in Mexican soils. Molecular Ecology 8:711-724.

Adriana Corvera, Danielle Promé, Jean-Claude Promé, Esperanza Martínez-Romero and David Romero. The nolL gene from Rhizobium etli determines nodulation efficiency by mediating the acetylation of the fucosyl residue in the nodulation factor. Molecular Plant-Microbe Interactions 12:236-246.

Jesús Caballero-Mellado and Esperanza Martínez-Romero. Soil fertilization limits the genetic diversity of Rhizobium in bean nodules. Symbiosis 26:111-121. Citas 16

E.T. Wang, P. van Berkum, X.H. Sui, D. Beyene, W.X. Chen and E. Martínez-Romero. Diversity of rhizobia associated with Amorpha fruticosa isolated from Chinese soils and description of Mesorhizobium amorphae sp. nov. International Journal of Systematic Bacteriology 49:51-65.


W. Ludwig, R. Amann, E. Martínez-Romero, W. Schönhuber, S. Bauer, A. Neef and K.-H. Schleifer. rRNA based identification and detection systems for rhizobia and other bacteria. In: Molecular Microbial Ecology of the Soil, G. Hardarson y W. Broughton (eds) Plant and Soil 204:1-19.

Margarita Flores, Patrick Mavingui, Lourdes Girard, Xavier Perret, William J. Broughton, Esperanza Martínez-Romero, Guillermo Dávila and Rafael Palacios. Three replicons of Rhizobium sp. strain NGR234 harbor symbiotic gene sequences. Journal of Bacteriology 180:6052-6053.

J. Vásquez-Arroyo, A. Sessitsch, E. Martínez and J.J. Peña-Cabriales. Nitrogen fixation and nodule occupancy by native strains of Rhizobium on different cultivars of common bean (Phaseolus vulgaris L.). In: Molecular Microbial Ecology of the Soil, G. Hardarson y W. Broughton (eds.) Plant and Soil 204:147-154.

E. Martínez-Romero, I. Hernández-Lucas, J.J. Peña- Cabriales and J.Z. Castellanos. Symbiotic performance of some modified Rhizobium etli strains in assays with Phaseolus vulgaris beans that have a high capacity to fix N2. In: Molecular Microbial Ecology of the Soil, G. Hardarson y W. Broughton (eds) Plant and Soil 204:89-94.

Patrick Mavingui, Toon Laeremans, Margarita Flores, David Romero, Esperanza Martínez-Romero and Rafael Palacios. Genes essential for Nod factor production and nodulation are located on a symbiotic amplicon (AMP RtrCFN299pc60) in Rhizobium tropici. Journal of Bacteriology 180:2866-2874.


L.L. Barrera, M.E. Trujillo, M. Goodfellow, F.J. García, I. Hernández-Lucas, G. Dávila, P. van Berkum and E. Martínez-Romero. Biodiversity of Bradyrhizobia nodulating Lupinus spp. International Journal of Systematic Bacteriology 47:1086-1091.

T. Jiménez-Salgado, L.E. Fuentes-Ramírez, A. Tapia-Hernández, M.A. Mascarúa-Esparza, E. Martínez-Romero and J. Caballero-Mellado. Coffea arabica L., a new host plant for Acetobacter diazotrophicus, and isolation of other nitrogen-fixing acetobacteria. Applied and Environmental Microbiology 63:3676-3683.

Z.Y. Tan, X.X. Xu, E.T. Wang, J.L. Gao, E. Martínez-Romero and W.X. Chen. Phylogenetic and genetic relationships of Mesorhizobium tianshanense and related rhizobia. International Journal of Systematic Bacteriology 47:874-879.

P. Mavingui,M. Flores, D. Romero, E. Martínez-Romero and R. Palacios. Generation of Rhizobium strains with improved symbiotic properties by random DNA amplification (RDA).
Nature Biotechnology 15:564-569.


T. Laeremans, I. Caluwaerts, C. Verreth, M.A. Rogel, J. Vanderleyden and E. Martínez-Romero. Isolation and characterization of the Rhizobium tropici Nod factor sulfation genes. Molecular Plant-Microbe Interactions 9:492-500.

A. Mendoza, A. Leija, E. Martínez-Romero, G. Hernández, and J. Mora. The enhancement of ammonium assimilation in Rhizobium etli prevents nodulation of Phaseolus vulgaris. Molecular Plant-Microbe Interactions 8:584-592.

S.M. Lohrke, James H. Orf, E. Martínez-Romero, and M.J. Sadowsky. Host-controlled restriction of nodulation by Bradyrhizobium japonicum strains in serogroup 110. Applied and Environmental Microbiology 61:2378-2383.

I. Hernández-Lucas, L. Segovia, E. Martínez-Romero and S.G. Pueppke. Phylogenetic relationships and host range of Rhizobium spp. that nodulate Phaseolus vulgaris L. Applied and Environmental Microbiology 61:2775-2779.

E. Geniaux, M. Flores, R. Palacios and E. Martínez. Presence of megaplasmids in Rhizobium tropici and further evidence of differences between the two R. tropici subtypes. International Journal of Systematic Bacteriology 45:392-394.

Rémy Poupot, E. Martínez-Romero, N. Gautier, J.C. Promé. Wild type Rhizobium etli, a bean symbiont, produces acetyl-fucosylated, N-methylated, and carbamoylated nodulation factors.
Journal of Biological Chemistry 270:6050-6055.


V. I. Romanov and E. Martínez-Romero. Sucrose transport and hydrolysis in Rhizobium tropici. In: Symbiotic Nitrogen Fixation, P.H. Graham, M.J. Sadowsky y C.P. Vance (eds.), Kluwer Academic Publishers, The Netherlands, Plant and Soil 161:91-96.

Jesús Caballero-Mellado and Esperanza Martínez-Romero. Limited genetic diversity in the endophytic sugarcane bacterium Acetobacter diazotrophicus. Applied and Environmental Microbiology 60:1532-1537.

Marco A. Pardo, Jaime Lagúnez, Juan Miranda and Esperanza Martínez. Nodulating ability of Rhizobium tropici is conditioned by a plasmid-encoded citrate synthase. Molecular Microbiology 11:315-321.


R. Poupot, E. Martínez-Romero and J.C. Promé. Nodulation factors from Rhizobium tropici are sulfated or nonsulfated chitopentasaccharides containing an N-methyl-N-acylglucosaminyl terminus. Biochemistry 32:10430-10435.

Lorenzo Segovia, J. Peter W. Young, and Esperanza Martínez-Romero. Reclassification of American Rhizobium leguminosarum biovar phaseoli type I strains as Rhizobium etli sp. nov.
International Journal of Systematic Bacteriology 43:374-377.


Esperanza Martínez-Romero, Lorenzo Segovia, Fabio Martins Mercante, Avílio Antonio Franco, Peter Graham and Marco Aurelio Pardo. Rhizobium tropici: a novel species nodulating Phaseolus vulgaris L. beans and Leucaena sp. trees. International Journal of Systematic Bacteriology 41:417-426.


Esperanza Martínez-Romero and Mónica Rosenblueth. Increased bean (Phaseolus vulgaris L.) nodulation competitiveness of genetically modified Rhizobium strains. Applied and Environmental Microbiology 56:2384-2388.

Esperanza Martínez, David Romero and Rafael Palacios. The Rhizobium genome. Critical Reviews in Plant Sciences 9:59-93.


Esperanza Martínez, Rafael Palacios and Federico Sánchez. Nitrogen-fixing nodules induced by Agrobacterium tumefaciens harboring rhizobium phaseoli plasmids. Journal of Bacteriology 169:2828-2834.

Frederic Debellé, Charles Rosenberg, Jacques Vasse, Fabienne Maillet, Esperanza Martínez, Jean Dénarié and Georges Truchet. Assignment of symbiotic developmental phenotypes to common and specific nodulation (nod) genetic loci of Rhizobium meliloti. Journal of Bacteriology 168:1075-1086.


Esperanza Martínez, Marco A. Pardo, Rafael Palacios & Miguel Angel Cevallos. Reiteration of nitrogen gene sequences and specificity of Rhizobium in nodulation and nitrogen fixation in Phaseolus vulgaris. The Journal of General Microbiology, 131:1779-1786.



Martínez J, Negrete-Yankelevich S, Gómez Godinez L, Reyes J, Degli Esposti M, Martínez Romero E. (2016). Short-term evolution of rhizobial strains towards sustainability in agriculture. In: Microbial Models: From Environment to Industrial Sustainability. Susana Castro-Sowinski (Ed.) Springer ISBN 978-981-10-2554-9 Microorganims for sustainability. Vol. I


Ormeño-Orrillo Ernesto, Zúñiga Dávila Doris, Martínez-Romero Esperanza. (2015). Biodiversity of nitrogen-fixing nodule bacteria associated with Lima bean (Phaseolus lunatus L.) in its domestication centers. En: Phaseolus lunatus, Ferreira de Araujo AS et al (Eds). Nova Science Publishers, Inc. Pág. 91-102. ISBN: 978-1-63482-494-1


Ernesto Ormeño-Orrillo. Mariangela Hungria, Esperanza Martínez-Romero. (2013).
Capítulo 11 Dinitrogen fixing prokaryotes. In: The Prokaryotes – Prokaryotic Physiology and Biochemistry. E. Rosenberg et al. (Eds.) Springer-Verlag Berlin Heidelberg 2013 DOI 10.1007/978-3-642-30141-4_72,

López-Guerrero, M.G., Ramírez M.A., Martínez-Romero, E. (2013). Rhizobial genetic repertoire to inhabit legume and nonlegume rhizospheres. In: Molecular Microbial Ecology of the Rhizosphere. Vol. 1, Frans J. de Bruijn (Ed.) pp. 495-500. Wiley Blackwell.

Navarro-Noya, Y.E., Martí¬nez-Romero, E., Hernández-Rodríguez, C. (2013). Potential plant-growth-promoting and nitrogen-fixing bacteria associated with pioneer plants growing on mine tailings. In: Molecular Microbial Ecology of the Rhizosphere. Vol. 2. Frans J. de Bruijn (Ed.). pp. 1003-1011. Wiley Blackwell 2013.